Complexities of biotic-abiotic interactions in soils result in the lack of integrated understanding of environmental variables that restrict the survival of shiga toxin-producing E. coli O157:H7. Herein, we reanalyzed previously published data and highlighted the influence of soil abiotic factors on E. coli O157:H7 survivability and elucidated how these factors took effect indirectly through affecting indigenous bacterial community. Interaction network analysis indicated salinity and pH decreased the relative abundances of some bacterial taxa (e.g., Acidobacteria_Gp4, Acidobacteria_Gp6, and Deltaproteobacteria) which were positively correlated with the survival of E. coli O157:H7 in soils, and vice versa (e.g., Gammaproteobacteria and Flavobacteria) (P < 0.05). An array of multivariate statistical approaches including partial Mantel test, variation partition analysis (VPA), and structural equation model (SEM) further confirmed that biotic and abiotic factors interactively shaped the survival profile of E. coli O157:H7. This study revealed that some bacterial taxa were correlated with survival of E. coli O157:H7 directly, and salinity and pH could affect E. coli O157:H7 survival through changing these bacterial taxa. These findings suggest that salinity in soil might benefit the control of fecal pathogenic E. coli invasion, while soil acidification caused by anthropogenic influences could potentially increase the persistence of E. coli O157:H7 in agro-ecosystem.

中文翻译：

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多变量统计显示，土壤盐度，pH和本地细菌群落相互作用影响大肠杆菌O157：H7的存活。

土壤中非生物-非生物相互作用的复杂性导致对环境变量缺乏全面的了解，这些环境变量限制了生产志贺毒素的大肠杆菌O157：H7的存活。在这里，我们重新分析了以前发表的数据，并强调了土壤非生物因素对大肠杆菌O157：H7生存能力的影响，并阐明了这些因素如何通过影响本地细菌群落间接发挥作用。相互作用网络分析表明，盐度和pH值降低了某些细菌类群（例如，Aceobacteria_Gp4，Acidobacteria_Gp6和Deltaproteobacteria）的相对丰度，这与土壤中大肠杆菌O157：H7的存活呈正相关，反之亦然（例如，γ-变形杆菌和黄杆菌（P <0.05）。一系列多元统计方法，包括部分Mantel检验，变异分区分析（VPA）和结构方程模型（SEM）进一步证实，生物因子和非生物因子相互作用地共同影响了O157：H7大肠杆菌的存活情况。这项研究表明，某些细菌类群与大肠杆菌O157：H7的存活率直接相关，盐度和pH值可能会通过改变这些细菌类群而影响大肠杆菌O157：H7的存活率。这些发现表明，土壤中的盐分可能有利于控制粪便病原性大肠杆菌的入侵，而人为因素引起的土壤酸化可能会增加大肠杆菌O157：H7在农业生态系统中的持久性。这项研究表明，某些细菌类群与大肠杆菌O157：H7的存活率直接相关，盐度和pH值可能会通过改变这些细菌类群而影响大肠杆菌O157：H7的存活率。这些发现表明，土壤中的盐分可能有利于控制粪便病原性大肠杆菌的入侵，而人为因素引起的土壤酸化可能会增加大肠杆菌O157：H7在农业生态系统中的持久性。这项研究表明，某些细菌类群与大肠杆菌O157：H7的存活率直接相关，盐度和pH值可能会通过改变这些细菌类群而影响大肠杆菌O157：H7的存活率。这些发现表明，土壤中的盐分可能有利于控制粪便病原性大肠杆菌的入侵，而人为因素引起的土壤酸化可能会增加大肠杆菌O157：H7在农业生态系统中的持久性。