The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was proposed. Measurements were carried out with red blood cells suspended in saline solution with human serum albumin at different concentrations. Erythrocytes adhered to a glass surface were subjected to different low shear stress (from 0.04 to 0.25 Pa), causing cellular deformation and dissociation. The maximum value obtained of the erythrocyte deformability index was 0.3, and that of the adhesion energy per unit area was 1.1 × 10⁻⁶ Pa m, both according to previous works. The obtained images of RBCs adhered to glass reveal that the adhesion is stronger in a single point of the cell, suggesting a ligand migration that concentrates the adhesion in a “spike-like tip” in the cell. Moreover, adhesion energy results indicate that the energy required to separate erythrocytes in media with a lower albumin concentration is greater. Both results could be explained by the mobility of membrane receptors.

通过微流体装置同时测定红细胞的粘附性和变形参数，该装置使用倒置光学显微镜和新的图像采集和分析技术。另外，提出了描述红细胞粘附和变形的模型的更新。用悬浮在盐溶液中的红细胞和不同浓度的人血清白蛋白进行测量。粘附在玻璃表面的红细胞受到不同的低剪切应力（0.04至0.25 Pa），导致细胞变形和离解。红细胞变形指数的最大值为0.3，每单位面积的粘附能的最大值为1.1×10 ^-6 Pa m，均根据先前的作品。获得的粘附在玻璃上的RBC图像显示，粘附力在细胞的单点上更强，表明配体迁移使粘附力集中在细胞的“尖峰状尖端”中。此外，粘附能结果表明，分离具有较低白蛋白浓度的培养基中的红细胞所需的能量更大。两种结果都可以用膜受体的迁移率来解释。