Seriola rivoliana intestinal microbiota (IM) was characterised under aquaculture conditions through 16S rRNA amplicon sequencing. Specimens of 30 days after hatching (DAH) were maintained in three tanks and fed under the same environmental conditions for characterisation 15 days prior to sampling. Three fish were randomly taken from each tank; total DNA extraction of the gut microbiota was performed to characterise microbial composition and its metabolic prediction. The V3 hypervariable region of the 16S rRNA was amplified and sequenced with Illumina pair-end technology. The prokaryotic components in the S. rivoliana intestine were dominated mainly by the phyla Proteobacteria, Firmicutes, Bacteroidetes, Cyanobacteria and Actinobacteria. No significant differences in beta diversity were detected in the three samples (tanks). However in alpha diversity, they were detected in juveniles of the same cohort within the same group, as exemplified by enrichment of certain bacterial groups, mainly of the Clostridia class, which were specific in each fish within the same tank. The metabolic prediction analyses suggested that S. rivoliana IM contribute to the metabolism of amino acids, carbohydrates, lipids, and immune system. This study provides the first IM characterisation under rearing conditions of S. rivoliana—a species with broad economic potential—and contributes to novel information for potential use of probiotics in future trials.

通过16S rRNA扩增子测序在水产养殖条件下鉴定了Seriola rivoliana肠道菌群（IM）。将孵化后30天（DAH）的样品保存在三个水箱中，并在取样前15天在相同的环境条件下进料进行表征。从每个水箱中随机抽取三条鱼；对肠道菌群进行总DNA提取以表征微生物组成及其代谢预测。16S rRNA的V3高变区通过Illumina末端技术进行了扩增和测序。rivoliana肠中的原核成分主要由变形杆菌，菌毛，拟杆菌，蓝细菌和放线细菌。在三个样品（储罐）中未检测到β多样性的显着差异。但是，在α多样性中，它们是在同一组的同一队列的幼鱼中检测到的，例如富集某些细菌组（主要是梭菌属）的示例，这些细菌组在同一缸中的每条鱼中都是特异性的。代谢预测分析表明，S。rivoliana IM有助于氨基酸，碳水化合物，脂质和免疫系统的代谢。这项研究提供了S. rivoliana饲养条件下的第一个IM表征-具有广泛经济潜力的物种-并为在未来的试验中可能使用益生菌提供了新的信息。