Nutrient turnover in soils is strongly driven by soil properties, including clay mineral composition. One main nutrient is phosphorus (P), which is known to be easily immobilized in soil. Therefore, the specific surface characteristics of clay minerals might substantially influence P availability in soil and thus the microbial strategies for accessing P pools. We used a metagenomic approach to analyze the microbial potential to access P after 842 days of incubation in artificial soils with a clay mineral composition of either non-expandable illite (IL) or expandable montmorillonite (MT), which differ in their surface characteristics like soil surface area and surface charge. Our data indicate that microorganisms of the two soils developed different strategies to overcome P depletion, resulting in similar total P concentrations. Genes predicted to encode inorganic pyrophosphatase (ppa), exopolyphosphatase (ppx), and the pstSCAB transport system were higher in MT, suggesting effective P uptake and the use of internal poly-P stores. Genes predicted to encode enzymes involved in organic P turnover like alkaline phosphatases (phoA, phoD) and glycerophosphoryl diester phosphodiesterase were detected in both soils in comparable numbers. In addition, P_o concentrations did not differ significantly. Most identified genes were assigned to microbial lineages generally abundant in agricultural fields, but some were assigned to lineages known to include oligotrophic specialists, such as Bacillaceae and Microchaetaceae.

土壤中的养分周转受土壤性质（包括粘土矿物成分）的强烈驱动。一种主要的养分是磷 (P)，众所周知，它很容易固定在土壤中。因此，粘土矿物的特定表面特征可能会显着影响土壤中磷的有效性，从而影响微生物获取磷库的策略。我们使用宏基因组方法来分析在人造土壤中培养 842 天后微生物获取磷的潜力，其中粘土矿物成分为不可膨胀伊利石 (IL) 或可膨胀蒙脱石 (MT)，它们的表面特征不同，如土壤表面积和表面电荷。我们的数据表明，两种土壤的微生物开发了不同的策略来克服 P 消耗，导致总 P 浓度相似。ppa )、外多磷酸酶 ( ppx ) 和pstSCAB转运系统在 MT 中较高，表明有效的 P 吸收和内部多 P 储存的使用。在两种土壤中检测到的基因预测编码参与有机磷周转的酶，如碱性磷酸酶（phoA、phoD）和甘油磷酰二酯磷酸二酯酶的数量相当。此外，P _o浓度没有显着差异。大多数已鉴定的基因被分配到通常在农田中丰富的微生物谱系，但一些被分配到已知包括寡营养专家的谱系，例如芽孢杆菌科和微毛菌科。