The development of metal nanoparticles (MNP) combined with a metal-organic framework (MOF) has received more and more attention due to its excellent synergistic catalytic ability, which can effectively broaden the scope of catalytic reactions and enhance the catalytic ability. In this work, we developed a novel ternary nanocomposite named Cu₂O-mediated Au nanoparticle (Au NP) grown on MIL-53(Fe) for real-time monitoring of hydrogen peroxide (H₂O₂) released from living cells. First, Cu₂O-MIL-53(Fe) was prepared by redox assembly technology, which provided the growth template, and active sites for AuCl⁴⁻. Au@Cu₂O-MIL-53(Fe)/GCE biosensor was prepared by further loading nano-Au uniformly on the surface of Cu₂O by electrochemical deposition. Compared to individual components, the hybrid nanocomposite showed superior electrochemical properties as electrode materials due to the synergistic effect between AuNPs, Cu₂O, and MIL-53(Fe). Electrochemical measurement showed that the Au@Cu₂O-MIL-53(Fe)/GCE biosensor presented a satisfactory catalytic activity towards H₂O₂ with a low detection limit of 1.01 μM and sensitivity of 351.57 μA mM⁻¹ cm⁻² in the linear range of 10–1520 μM. Furthermore, this biosensor was successfully used for the real-time monitoring of dynamic H₂O₂ activated by PMA released from living cells. And the great results of confocal fluorescence microscopy of the co-culture cells with PMA and Au@Cu₂O-MIL-53(Fe) verified the reliability of the biosensor, suggesting its potential application to the monitoring of critical pathological processes at the cellular level.

金属纳米粒子（MNP）与金属有机骨架（MOF）结合的开发因其优异的协同催化能力而受到越来越多的关注，可有效拓宽催化反应范围，增强催化能力。在这项工作中，我们开发了一种名为 Cu ₂ O 介导的 Au 纳米颗粒 (Au NP)的新型三元纳米复合材料，该颗粒生长在 MIL-53(Fe) 上，用于实时监测活细胞释放的过氧化氢 (H ₂ O ₂ )。首先，通过氧化还原组装技术制备Cu ₂ O-MIL-53(Fe)，为AuCl ^4-提供生长模板和活性位点。Au@Cu ₂O-MIL-53(Fe)/GCE 生物传感器是通过电化学沉积进一步将纳米金均匀负载在 Cu ₂ O表面上制备的。与单个组件相比，由于 AuNP、Cu ₂ O 和 MIL-53(Fe)之间的协同作用，混合纳米复合材料作为电极材料显示出优异的电化学性能。电化学测量表明，Au@Cu ₂ O-MIL-53(Fe)/GCE 生物传感器对 H ₂ O ₂表现出令人满意的催化活性，低检测限为 1.01 μM，灵敏度为 351.57 μA mM ^-1  cm ^-2 in 10–1520 μM 的线性范围。此外，该生物传感器已成功用于动态 H 的实时监测。₂ O ₂被活细胞释放的PMA激活。与 PMA 和 Au@Cu ₂ O-MIL-53(Fe)共培养细胞的共聚焦荧光显微镜的良好结果验证了生物传感器的可靠性，表明其在监测细胞关键病理过程中的潜在应用。等级。