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Reclassification of Chromobacterium violaceum ATCC 31532 and its quorum biosensor mutant CV026 to Chromobacterium subtsugae
AMB Express ( IF 3.7 ) Pub Date : 2020-11-07 , DOI: 10.1186/s13568-020-01140-1
Alisha M. Harrison , Scott D. Soby

The precipitous drop in the cost of genomic sequencing and the concomitant availability of computational methods for comparing genome-level data has made the accurate taxonomic placement of bacteria affordable and relatively rapid. Inaccurate taxonomic placement of bacteria has serious implications in clinical, environmental, and regulatory microbiology, but it can also adversely affect interpretation of research results. The quorum biosensor strain CV026 was derived from an isolate of Chromobacterium that was labeled as C. violaceum ATCC 31532, and is catalogued by the ATCC under that species name. Nearly 200 papers have been published that use CV026 as an indicator for quorum sensing activity in many Gram negative bacteria, but the inability of C. violaceum strains to complement the quorum sensing mutation in CV026 has called the taxonomic placement of the parent strain into question. We used molecular phylogeny and a large number of metabolic and phenotypic characters to demonstrate that Chromobacterium strain ATCC 31532 is a member of species Chromobacterium subtsugae.



中文翻译:

紫罗兰色杆菌ATCC 31532及其法定生物传感器突变体CV026重新分类为Subtsugae杆菌

基因组测序成本的急剧下降以及用于比较基因组水平数据的计算方法的随之而来,使得细菌的准确分类学放置负担得起且相对较快。细菌分类分类的不正确对临床,环境和法规微生物学有严重影响,但也会对研究结果的解释产生不利影响。仲裁生物传感器应变CV026从分离株衍生的色杆菌的是标为C.堇色ATCC 31532,并且通过根据该物种名称ATCC编目。已经发表了近200篇论文,这些论文使用CV026作为许多革兰氏阴性细菌的群体感应活动的指标,但是无力C. violaceum补充CV026中群体感应突变的菌株已将亲本菌株的分类放置问题引起了质疑。我们使用分子系统发生和大量的代谢和表型特征证明色杆菌菌株ATCC 31532是物种成员色杆菌subtsugae

更新日期:2020-11-09
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