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Association between genetic polymorphisms of telomere pathway genes and hydrogen peroxide level in omethoate exposure workers
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-11-05 , DOI: 10.1016/j.etap.2020.103541
Xiaoshan Zhou , Xinling Li , Wan Wei , Xiaoran Duan , Hui Zhang , Mingcui Ding , Wu Yao , Qi Wang , Wei Wang , Yongli Yang

Objective

The aim of this study was to explore the association between genetic variations in telomere pathway genes and the level of hydrogen peroxide (H2O2) in omethoate exposure workers.

Methods

A total of 180 omethoate exposure workers and 115 healthy controls were recruited. The level of H2O2 in plasma was determined with molybdenic acid colorimetry. Polymerase chain reaction and restriction fragment length was used to detect polymorphisms in POT1 rs1034794, POT1 rs10250202, TERF1 rs3863242, and TERT rs2736098.

Results

The level of H2O2 in exposure group (4.26 ± 0.71) was significantly higher than that in control group (3.29 ± 0.46). Generalized linear models indicated that risk factors for the increase H2O2 level were exposure [β(95 % CI) = 0.951 (0.806, 1.096), P < 0.001] and AA + AT genotype in POT1 rs034794 [β(95 % CI) = 0.397 (0.049, 0.745), P = 0.025].

Conclusion

The increase H2O2 level was associated with omethoate exposure and AA + AT genotypes in POT1 gene rs1034794. It provided a new idea that polymorphisms in telomere pathway genes may indirectly regulate telomere length by influencing oxidative stress.



中文翻译:

氧化乐果暴露工人端粒途径基因遗传多态性与过氧化氢水平的关系

目的

这项研究的目的是探讨氧化乐果暴露工人端粒途径基因的遗传变异与过氧化氢(H 2 O 2)水平之间的关系。

方法

总共招募了180名氧化乐果暴露工人和115位健康对照。用钼酸比色法测定血浆中的H 2 O 2含量。聚合酶链反应和限制性片段长度用于检测POT1 rs1034794,POT1 rs10250202,TERF1 rs3863242和TERT rs2736098中的多态性。

结果

暴露组H 2 O 2的水平(4.26±0.71)显着高于对照组(3.29±0.46)。广义线性模型表明,H 2 O 2水平升高的危险因素是暴露[β(95%CI)= 0.951(0.806,1.096),P <0.001]和POT1 rs034794中AA + AT基因型[β(95%CI) )= 0.397(0.049,0.745),P = 0.025]。

结论

H 2 O 2水平升高与氧化乐果暴露和POT1基因rs1034794中的AA + AT基因型有关。它提供了一个新的想法,即端粒途径基因的多态性可能通过影响氧化应激而间接调节端粒的长度。

更新日期:2020-11-26
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