Enzymes play a key role in many bioprocesses associated with diseases, and observation of enzyme dynamics in living bodies has become meaningful for understanding enzyme function. Thus, in situ real-time fluorescence imaging can prove to be a powerful tool for monitoring enzyme dynamics, enabling detailed understanding of changes in the activity and function of enzymes. This perspective summarizes the existing methods for in situ real-time fluorescence imaging of enzymes in vivo and discusses remaining challenges. This perspective is intended to encourage chemists and chemical engineers to develop additional methods for the in situ real-time monitoring of enzymes in complex biosystems.

酶在与疾病有关的许多生物过程中都起着关键作用，观察生物体内酶的动态对于理解酶的功能已变得有意义。因此，原位实时荧光成像可以证明是监测酶动力学的强大工具，可以详细了解酶活性和功能的变化。此透视图概括了用于现有方法的原位酶的实时荧光成像体内和剩余的挑战讨论。此观点旨在鼓励化学家和化学工程师开发用于复杂生物系统中酶的原位实时监测的其他方法。