Chem
Volume 6, Issue 11, 5 November 2020, Pages 2893-2901
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Perspective
Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes

https://doi.org/10.1016/j.chempr.2020.10.010Get rights and content
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The Bigger Picture

Challenges and opportunities:

  • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem

  • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge

  • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes

Summary

Enzymes play a key role in many bioprocesses associated with diseases, and observation of enzyme dynamics in living bodies has become meaningful for understanding enzyme function. Thus, in situ real-time fluorescence imaging can prove to be a powerful tool for monitoring enzyme dynamics, enabling detailed understanding of changes in the activity and function of enzymes. This perspective summarizes the existing methods for in situ real-time fluorescence imaging of enzymes in vivo and discusses remaining challenges. This perspective is intended to encourage chemists and chemical engineers to develop additional methods for the in situ real-time monitoring of enzymes in complex biosystems.

UN Sustainable Development Goals

SDG3: Good health and well-being

Keywords

enzymes
dynamics
in situ
real-time
fluorescence imaging

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