Salinity is one of the most vicious environmental constraints that hamper agricultural production. Experiments were done to explore the significant role of sole and synergistic supplementation of kinetin (100 µM KN) and putrescine (100 µM PUT) on Luffa acutangula in NaCl (100 mM) treatment. The harmful effects of salinity on growth were manifested by decreased seedling length, biomass, and pigment contents. We studied the effect of KN, and PUT in preventing salt (NaCl) induced physiological disorders and oxidative damages in 20-day-old Luffa acutangula seedlings. The individual application of KN and PUT increased growth and biochemical parameters, whereas combined KN + PUT treatment showed significant enhancement in growth, photosynthetic pigment content, and osmolyte accumulation in salt-affected plants. Application of KN and PUT also prevented hydrogen peroxide and superoxide production as confirmed by inhibition in electrolyte leakage and lipid peroxidation. Kinetin and PUT application upregulated the antioxidant defense system by enhancing antioxidant enzymes and non-enzymatic contents. Luffa seedlings treated with NaCl + KN + PUT showed 79, 26, 74, and 73% rise in superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase enzymes, respectively, in comparison to NaCl-stressed Luffa acutangula. Findings revealed that synergistic utilization of KN and PUT modulate growth and biochemical processes in seedlings efficaciously in comparison to the individual application under salt stress, and it may be due to a regulatory crosstalk mechanism.

盐度是阻碍农业生产的最恶劣的环境制约因素之一。进行了实验以探索在 NaCl (100 mM) 处理中单独和协同补充激动素 (100 µM KN) 和腐胺 (100 µM PUT) 对丝瓜的重要作用。盐度对生长的有害影响表现为幼苗长度、生物量和色素含量降低。我们研究了 KN 和 PUT 在预防 20 日龄丝瓜中盐 (NaCl) 诱导的生理障碍和氧化损伤方面的作用幼苗。KN 和 PUT 的单独应用增加了生长和生化参数，而 KN + PUT 的组合处理显示出受盐影响植物的生长、光合色素含量和渗透物积累显着增强。KN 和 PUT 的应用还可以防止过氧化氢和超氧化物的产生，这通过抑制电解质泄漏和脂质过氧化得到证实。激动素和 PUT 应用通过增强抗氧化酶和非酶含量来上调抗氧化防御系统。丝瓜用NaCl + KN + PUT处理的秧苗显示79，26，74，和73％的增长在超氧化物歧化酶，过氧化氢酶，抗坏血酸过氧化物酶和谷胱甘肽还原酶，分别在比较NaCl的强调棱角丝瓜. 研究结果表明，与盐胁迫下的单独应用相比，KN 和 PUT 的协同利用可有效调节幼苗的生长和生化过程，这可能是由于调节性串扰机制。