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Exogenous kinetin and putrescine synergistically mitigate salt stress in Luffa acutangula by modulating physiology and antioxidant defense

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Abstract

Salinity is one of the most vicious environmental constraints that hamper agricultural production. Experiments were done to explore the significant role of sole and synergistic supplementation of kinetin (100 µM KN) and putrescine (100 µM PUT) on Luffa acutangula in NaCl (100 mM) treatment. The harmful effects of salinity on growth were manifested by decreased seedling length, biomass, and pigment contents. We studied the effect of KN, and PUT in preventing salt (NaCl) induced physiological disorders and oxidative damages in 20-day-old Luffa acutangula seedlings. The individual application of KN and PUT increased growth and biochemical parameters, whereas combined KN + PUT treatment showed significant enhancement in growth, photosynthetic pigment content, and osmolyte accumulation in salt-affected plants. Application of KN and PUT also prevented hydrogen peroxide and superoxide production as confirmed by inhibition in electrolyte leakage and lipid peroxidation. Kinetin and PUT application upregulated the antioxidant defense system by enhancing antioxidant enzymes and non-enzymatic contents. Luffa seedlings treated with NaCl + KN + PUT showed 79, 26, 74, and 73% rise in superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase enzymes, respectively, in comparison to NaCl-stressed Luffa acutangula. Findings revealed that synergistic utilization of KN and PUT modulate growth and biochemical processes in seedlings efficaciously in comparison to the individual application under salt stress, and it may be due to a regulatory crosstalk mechanism.

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Acknowledgements

Dr. Kapoor is grateful to Amity Institute of Biotechnology, Amity University Uttar Pradesh, for providing the laboratory facilities to carry out the work.

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  1. Plant Physiology Laboratory, Amity Institute of Biotechnology, Amity University, Noida, 201 313, Uttar Pradesh, India

    Riti Thapar Kapoor

  2. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman

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  1. Riti Thapar Kapoor
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RTK and MH designed and planned the experiment. RTK conducted the experiment. MH analyzed the data. RTK and MH prepared the manuscript draft. MH edited the manuscript. Both authors approved the final version of the manuscript.

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Correspondence to Mirza Hasanuzzaman.

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Kapoor, R.T., Hasanuzzaman, M. Exogenous kinetin and putrescine synergistically mitigate salt stress in Luffa acutangula by modulating physiology and antioxidant defense. Physiol Mol Biol Plants 26, 2125–2137 (2020). https://doi.org/10.1007/s12298-020-00894-z

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