Abstract Due to the rise of antifungal resistance and emerging fungal pathogens, there is an increasing need to find efficient treatment for Candida infections. This study determined the inhibition of combinational therapy against two of the most frequently found Candida strains in the oral cavity, C. albicans and C. krusei. Minimal inhibitory concentrations of amphotericin B, fluconazole, and 5-fluorocytosine and the effect of combination therapy were determined for these strains by isothermal microcalorimetry. Fluconazole was the most effective antimicrobial at the concentrations included. Additionally, adding fluconazole to the other two antimicrobials rapidly inhibited the growth of both Candida species. Thus, the microcalorimetric technique is not only well-fitted for in vitro testing of drug combinations for treatment, but also for applications in drug development.

中文翻译：

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等温微量热测定抗真菌剂对念珠菌的抑制作用

摘要 由于抗真菌耐药性的增加和新出现的真菌病原体，越来越需要寻找有效的念珠菌感染治疗方法。该研究确定了联合疗法对口腔中两种最常见的念珠菌菌株白色念珠菌和克柔念珠菌的抑制作用。通过等温微量热法测定这些菌株的两性霉素 B、氟康唑和 5-氟胞嘧啶的最小抑制浓度以及联合治疗的效果。氟康唑在所包括的浓度下是最有效的抗菌剂。此外，在其他两种抗菌剂中加入氟康唑可迅速抑制两种念珠菌的生长。因此，微量热技术不仅非常适合用于治疗药物组合的体外测试，