Elsevier

Thermochimica Acta

Volume 694, December 2020, 178806
Thermochimica Acta

Isothermal microcalorimetric determination of the inhibition of Candida species by antifungals

https://doi.org/10.1016/j.tca.2020.178806Get rights and content
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Highlights

  • Isothermal microcalorimetry is fast and sensitive, allowing rapid antifungal drug susceptibility testing.

  • Tri-plots are an ideal way to present results from drug combinations.

  • Demonstrated method allows effect of combinations with limited number of measurements.

Abstract

Due to the rise of antifungal resistance and emerging fungal pathogens, there is an increasing need to find efficient treatment for Candida infections. This study determined the inhibition of combinational therapy against two of the most frequently found Candida strains in the oral cavity, C. albicans and C. krusei. Minimal inhibitory concentrations of amphotericin B, fluconazole, and 5-fluorocytosine and the effect of combination therapy were determined for these strains by isothermal microcalorimetry. Fluconazole was the most effective antimicrobial at the concentrations included. Additionally, adding fluconazole to the other two antimicrobials rapidly inhibited the growth of both Candida species. Thus, the microcalorimetric technique is not only well-fitted for in vitro testing of drug combinations for treatment, but also for applications in drug development.

Keywords

Candida
Isothermal microcalorimetry
Resistance
Antifungal therapy
Tri-plot

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