Cetyl palmitate was produced by the esterification of palmitic acid with cetyl alcohol using n-hexane as solvent and a fermented solid of Burkholderia contaminans as catalyst. Effects of temperature (35–55 °C) and alcohol:acid molar ratio (0.5:1–2:1) on the reaction kinetics were evaluated for a fixed amount of catalyst (10 wt% related to the total mass of substrates) and a stirring speed (600 rpm) that was sufficiently high to avoid convective mass transfer limitations in the bulk phase. The temperature did not to influence the reaction rates and conversions above 90% were obtained for all temperatures after 12 h of reaction using a stoichiometric ratio (1:1) between the substrates. The prepared catalyst was recycled and reused in seven consecutive reactions of 8 h each and lost around 40% of its initial activity during the process. A Ping-Pong Bi–Bi kinetic model was proposed to represent the system reactions and was satisfactorily fitted to the experimental data.

棕榈酸十六酯是由棕榈酸的酯化而制备用鲸蜡醇Ñ作为溶剂正己烷和发酵固体的伯克霍尔德contaminans作为催化剂。对于固定量的催化剂（相对于底物总质量为10 wt％），评估了温度（35–55°C）和醇：酸摩尔比（0.5：1-2–1）对反应动力学的影响，足够高的搅拌速度（600 rpm）可以避免本体相中对流传质的限制。温度不影响反应速率，并且在反应12小时后，使用底物之间的化学计量比（1：1），所有温度下的转化率均高于90％。所制备的催化剂被循环使用，并在每个连续8小时的七个连续反应中重复使用，并在此过程中损失了其初始活性的约40％。提出了一种乒乓Bi-Bi动力学模型来代表系统反应，并令人满意地拟合了实验数据。