The development of nanoparticle probes has opened up new possibilities for molecular imaging in the era of precision medicine. There are a wide range of nanoprobes that are being used for various modalities that have demonstrated promising potential in early detection, disease monitoring, and theranostics. However, the rate of successful clinical translation of the nanoprobes is very low and is affected by the lack of our understanding about nanoparticle interaction with biological fluids after systemic administration, thus representing an unmet clinical need. One of the poorly understood issues relates to the formation of biomolecular corona, a layer of biomolecules formed on the surface of nanoscale materials during their interactions with biological fluids. The biomolecular corona has several significant effects on the biodistribution of nanoprobes and their imaging ability by (i) reducing their targeting efficacy and (ii) affecting the intrinsic imaging properties (e.g., contrast capacity of magnetic nanoprobes). This review provides insights on the importance of considering biomolecular corona in the development of nanoprobes, which may enable their more efficient utilization for molecular imaging applications.

纳米粒子探针的发展为精准医学时代的分子成像开辟了新的可能性。有广泛的纳米探针被用于各种模式，这些模式在早期检测、疾病监测和治疗诊断方面显示出巨大的潜力。然而，纳米探针的成功临床转化率非常低，并且受到我们对全身给药后纳米粒子与生物体液相互作用缺乏了解的影响，因此代表了未满足的临床需求。一个鲜为人知的问题与生物分子电晕的形成有关，这是在纳米级材料与生物流体相互作用期间在纳米级材料表面形成的一层生物分子。例如，磁性纳米探针的对比能力）。本综述提供了关于在纳米探针开发中考虑生物分子电晕的重要性的见解，这可能使它们更有效地用于分子成像应用。