Natural rubber or latex from the Hevea brasiliensis is an important commodity in various economic sectors in today’s modern society. Proteins have been detected in latex since the early twentieth century, and they are known to regulate various biological pathways within the H. brasiliensis trees such as the natural rubber biosynthesis, defence against pathogens, wound healing, and stress tolerance. However, the exact mechanisms of the pathways are still not clear. Proteomic analyses on latex have found various proteins and revealed how they fit into the mechanisms of the biological pathways. In the past three decades, there has been rapid latex protein identification due to the improvement of latex protein extraction methods, as well as the emergence of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). In this manuscript, we reviewed the methods of latex protein extraction that keeps on improving over the past three decades as well as the results of numerous latex protein identification and quantitation.

来自巴西橡胶树的天然橡胶或乳胶是当今现代社会各个经济部门的重要商品。自二十世纪初以来，人们就在乳胶中检测到了蛋白质，并且已知它们可以调节H. brasiliensis内的各种生物途径天然橡胶的生物合成、抵御病原体、伤口愈合和压力耐受性等树木。然而，这些途径的确切机制仍不清楚。对乳胶的蛋白质组学分析发现了多种蛋白质，并揭示了它们如何适应生物途径的机制。在过去的三十年里，由于乳胶蛋白提取方法的改进，以及二维凝胶电泳（2-DE）和质谱（MS）的出现，乳胶蛋白的鉴定得到了快速的发展。在这篇手稿中，我们回顾了过去三十年来不断改进的乳胶蛋白提取方法，以及众多乳胶蛋白鉴定和定量的结果。