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Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward

  • Current Topics in Plant Research
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Abstract

Natural rubber or latex from the Hevea brasiliensis is an important commodity in various economic sectors in today’s modern society. Proteins have been detected in latex since the early twentieth century, and they are known to regulate various biological pathways within the H. brasiliensis trees such as the natural rubber biosynthesis, defence against pathogens, wound healing, and stress tolerance. However, the exact mechanisms of the pathways are still not clear. Proteomic analyses on latex have found various proteins and revealed how they fit into the mechanisms of the biological pathways. In the past three decades, there has been rapid latex protein identification due to the improvement of latex protein extraction methods, as well as the emergence of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). In this manuscript, we reviewed the methods of latex protein extraction that keeps on improving over the past three decades as well as the results of numerous latex protein identification and quantitation.

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References

  • Abd-Rahman N, Kamarrudin MF (2018) Proteomic profiling of Hevea latex serum induced by ethephon stimulation. J Trop Plant Physiol 10:11–22

    Google Scholar 

  • Agrawal AA, Konno K (2009) Latex: a model for understanding mechanisms, ecology, and evolution of plant defense against herbivory. Annu Rev Ecol Evol Syst 40:311–331

    Article  Google Scholar 

  • Amalou Z, Bangratz J, Chrestin H (1992) Ethrel (ethylene releaser)-induced increases in the adenylate pool and transtonoplast ΔpH within Hevea latex cells. Plant Physiol 98:1270–1276

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bottier C (2020) Biochemical composition of Hevea brasiliensis latex: a focus on the protein, lipid, carbohydrate and mineral contents. In: Robert N (ed) Adv Bot Res, vol 93. Academic Press, London, pp 201–237

    Google Scholar 

  • Chow KS, Wan KL, Isa MNM, Bahari A, Tan SH, Harikrishna K, Yeang HY (2007) Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex. J Exp Bot 58:2429–2440

    Article  CAS  PubMed  Google Scholar 

  • Chow KS, Mat-Isa MN, Bahari A, Ghazali AK, Alias H, Mohd-Zainuddin Z, Hoh CC, Wan KL (2012) Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. J Exp Bot 63:1863–1871

    Article  CAS  PubMed  Google Scholar 

  • Chunliu C, Jie Y, Zhi D, Shoucai C, Dejun L (2010) Differential analysis of 2-DE profiles and preliminary identification of lutoids proteins from TPD tree in Hevea brasiliensis. Chin Agric Sci Bull 26:304–308

    Google Scholar 

  • Cornish K, Backhaus RA (1990) Rubber transferase activity in rubber particles of guayule. Phytochemistry 29:3809–3813

    Article  CAS  Google Scholar 

  • Cornish K, Siler DJ (1996) Characterization of cis-prenyl transferase activity localized in a buoyant fraction of rubber particles from Ficus elastica latex. Plant Physiol Biochem 34:377–384

    CAS  Google Scholar 

  • Cornish K, Wood DF, Windle JJ (1999) Rubber particles from four different species, examined by transmission electron microscopy and electron-paramagnetic-resonance spin labeling, are found to consist of a homogeneous rubber core enclosed by a contiguous, monolayer biomembrane. Planta 210:85–96

    Article  CAS  PubMed  Google Scholar 

  • Cornish K, Scott DJ, Xie W, Mau CJ, Zheng YF, Liu XH, Prestwich GD (2018) Unusual subunits are directly involved in binding substrates for natural rubber biosynthesis in multiple plant species. Phytochemistry 156:55–72

    Article  CAS  PubMed  Google Scholar 

  • Coupé M, Lambert C (1977) Absorption of citrate by the lutoids of latex and rubber production by Hevea. Phytochemistry 16:455–458

    Article  Google Scholar 

  • Dai L, Xiang Q, Li Y, Nie Z, Kang G, Duan C, Zeng R (2012) Rubber particle protein analysis of Hevea brasiliensis by two dimensional 16-BAC/SDS-PAGE and mass spectrometry. Zhongguo Nong Ye Ke Xue 45:2328–2338

    CAS  Google Scholar 

  • Dai L, Kang G, Li Y, Nie Z, Duan C, Zeng R (2013) In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree). Plant Mol Biol 82:155–168

    Article  CAS  PubMed  Google Scholar 

  • Dai L, Kang G, Nie Z, Li Y, Zeng R (2015) Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC–MS/MS. J Proteom 132:167–175

    Article  Google Scholar 

  • D’Auzac J, Prévôt JC, Jacob C (1995) What’s new about lutoids? A vacuolar system model from Hevea latex. Plant Physiol Biochem 33:765–777

    CAS  Google Scholar 

  • Dejun L, Jie Y, Zhi D, Chunliu C, Peng H, Min W, Shoucai C (2009) Evaluation of three methods for protein extraction suitable for 2-DE of Hevea brasiliensis C-serum. Chin Agric Sci Bull 25:273–279

    Google Scholar 

  • Dennis MS, Light DR (1989) Rubber elongation factor from Hevea brasiliensis. Identification, characterization, and role in rubber biosynthesis. J Biol Chem 264:18608–18617

    Article  CAS  PubMed  Google Scholar 

  • Dian K, Sangare A, Diopoh JK (1995) Evidence for specific variation of protein pattern during tapping panel dryness condition development in Hevea brasiliensis. Plant Sci 105:207–216

    Article  CAS  Google Scholar 

  • Duan CF, Nie ZY, Zeng RZ (2006) Establishment of 2-DE system and primary analyses on the membrane proteins of rubber particles in Hevea brasiliensis by MALDI-TOF. Chin J Tropic Crops 27:22–29

    Google Scholar 

  • Faurobert M, Pelpoir E, Chaïb J (2007) Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues. In: Zivy M (ed) Plant proteomics, methods and protocols, vol 355. Humana Press, New Jersey, pp 9–14

    Google Scholar 

  • Gao L, Sun Y, Wu M, Wang D, Wei J, Wu B, Wang G, Wu W, Jin X, Wang X, He P (2018) Physiological and proteomic analyses of molybdenum-and ethylene-responsive mechanisms in rubber latex. Front Plant Sci 9:621

    Article  PubMed  PubMed Central  Google Scholar 

  • Gemperline E, Keller C, Li L (2016) Mass spectrometry in plant-omics. Anal Chem 88:3422–3434

    Article  CAS  PubMed  Google Scholar 

  • Gidrol X, Chrestin H, Tan HL, Kush ANIL (1994) Hevein, a lectin-like protein from Hevea brasiliensis (rubber tree) is involved in the coagulation of latex. J Biol Chem 269:9278–9283

    Article  CAS  PubMed  Google Scholar 

  • Habib MAH, Yuen GC, Othman F, Zainudin NN, Latiff AA, Ismail MN (2017) Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600). Biochem Cell Biol 95:232–242

    Article  CAS  PubMed  Google Scholar 

  • Habib MAH, Gan CY, Abdul Latiff A, Ismail MN (2018) Unrestrictive identification of post-translational modifications in Hevea brasiliensis latex. Biochem Cell Biol 96:818–824

    Article  CAS  PubMed  Google Scholar 

  • Havanapan PO, Bourchookarn A, Ketterman AJ, Krittanai C (2015) Comparative proteome analysis of rubber latex serum from pathogenic fungi tolerant and susceptible rubber tree (Hevea brasiliensis). J Proteomics 131:82–92

    Article  PubMed  Google Scholar 

  • Jacob JL, d’Auzac J, Prevot JC (1993) The composition of natural latex from Hevea brasiliensis. Clin Rev Allergy 11:325

    Article  CAS  PubMed  Google Scholar 

  • Kanokwiroon K, Teanpaisan R, Wititsuwannakul D, Hooper AB, Wititsuwannakul R (2008) Antimicrobial activity of a protein purified from the latex of Hevea brasiliensis on oral microorganisms. Mycoses 51:301–307

    Article  CAS  PubMed  Google Scholar 

  • Kekwick RGO (2018) The formation of isoprenoids in Hevea latex. In: d’Auzac J (ed) Physiology of rubber tree latex: The laticiferous cell and latex—a model of cytoplasm. CRC Press, Florida, pp 145–164

    Chapter  Google Scholar 

  • Kerche-Silva LE, Cavalcante DGSM, Danna CS, Gomes AS, Carrara IM, Cecchini AL, Yoshihara E, Job AE (2017) Free-radical scavenging properties and cytotoxic activity evaluation of latex C-serum from Hevea brasiliensis RRIM 600. Free Radic Antioxid 7:107–114

    Article  CAS  Google Scholar 

  • Ko JH, Chow KS, Han KH (2003) Transcriptome analysis reveals novel features of the molecular events occurring in the laticifers of Hevea brasiliensis (para rubber tree). Plant Mol Biol 53:479–492

    Article  CAS  PubMed  Google Scholar 

  • Konno K (2011) Plant latex and other exudates as plant defense systems: roles of various defense chemicals and proteins contained therein. Phytochemistry 72:1510–1530

    Article  CAS  PubMed  Google Scholar 

  • Li HL, Guo D, Lan FY, Tian WM, Peng SQ (2011) Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones. Acta Physiol Plant 33:1853–1859

    Article  CAS  Google Scholar 

  • Liengprayoon S, Vaysee L, Jantarasunthorn S, Wadeesirisak K, Chaiyut J, Srisomboon S, Musigamart N, Roytrakul S, Bonfilis F, Char C, Bottier C (2017) Fractionation of Hevea brasiliensis latex by centrifugation: (i) a comprehensive description of the biochemicalcomposition of the 4 centrifugation fractions. IRRDB 2017. Jakarta 1:645–660

    Google Scholar 

  • Martin MN (1991) The latex of Hevea brasiliensis contains high levels of both chitinases and chitinases/lysozymes. Plant Physiol 95:469–476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Men X, Wang F, Chen GQ, Zhang HB, Xian M (2019) Biosynthesis of natural rubber: current state and perspectives. Int J Mol Sci 20:50

    Article  Google Scholar 

  • Oh SK, Kang H, Shin DH, Yang J, Chow KS, Yeang HY, Wagner B, Breiteneder H, Han KH (1999) Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem 274:17132–17138

    Article  CAS  PubMed  Google Scholar 

  • Posch A, Chen Z, Wheeler C, Dunn MJ, Raulf-Heimsoth M, Baur X (1997) Characterization and identification of latex allergens by two-dimensional electrophoresis and protein microsequencing. J Allergy Clin Immunol 99:385–395

    Article  CAS  PubMed  Google Scholar 

  • Priyadarshan PM (2017) Latex production, diagnosis and harvest. In: Priyadarshan PM (ed) Biology of hevea rubber, 2nd edn. CAB International, Wallingford, pp 51–82

    Chapter  Google Scholar 

  • Rahman AYA, Usharraj AO, Misra BB, Thottathil GP, Jayasekaran K, Feng Y, Hou S, Ong SY, Ng FL, Lee LS, Tan HS (2013) Draft genome sequence of the rubber tree Hevea brasiliensis. BMC Genom 14:75

    Article  Google Scholar 

  • Sansatsadeekul J, Sakdapipanich J, Rojruthai P (2011) Characterization of associated proteins and phospholipids in natural rubber latex. J Biosci Bioeng 111:628–634

    Article  CAS  PubMed  Google Scholar 

  • Siler DJ, Goodrich-Tanrikulu M, Cornish K, Stafford AE, McKeon TA (1997) Composition of rubber particles of Hevea brasiliensis, Parthenium argentatum, Ficus elastica, and Euphorbia lactiflua indicates unconventional surface structure. Plant Physiol Biochem 35:881–889

    CAS  Google Scholar 

  • Singh AP, Wi SG, Chung GC, Kim YS, Kang H (2003) The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis. J Exp Bot 54:985–992

    Article  CAS  PubMed  Google Scholar 

  • Slomkowski S, Alemán JV, Gilbert RG, Hess M, Horie K, Jones RG, Kubisa P, Meisel I, Moemann W, Penczek S, Stepto RF (2011) Terminology of polymers and polymerization processes in dispersed systems (IUPAC Recommendations 2011). Pure Appl Chem 83:2229–2259

    Article  CAS  Google Scholar 

  • Spence D (1908) On the presence of oxydases in india-rubber, with a theory in regard to their function in the latex. Biochem J 3:165

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Srisomboon S, Wadeesirisak K, Sauvage FX, Rattanaporn K, Sriroth K, Vaysse L, Bonfils F, Sainte-Beuve J, Liengprayoon S, Bottier C (2014) Optimization of protein extraction from different latex samples of Hevea brasiliensis. Thaksin Univ J 17:26

    Google Scholar 

  • Subroto T, de Vries H, Schuringa JJ, Soedjanaatmadja UM, Hofsteenge J, Jekel PA, Beintema JJ (2001) Enzymic and structural studies on processed proteins from the vacuolar (lutoid-body) fraction of latex of Hevea brasiliensis. Plant Physiol Biochem 39:1047–1055

    Article  CAS  Google Scholar 

  • Tupý J (1973) The regulation of invertase activity in the latex of Hevea brasiliensis Muell. Arg: the effects of growth regulators, bark wounding, and latex tapping. J Exp Bot 24:516–524

    Article  Google Scholar 

  • Wang X, Shi M, Lu X, Ma R, Wu C, Guo A, Peng M, Tian W (2010) A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS. Proteome Sci 8:35

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang X, Shi M, Wang D, Chen Y, Cai F, Zhang S, Wang L, Tong Z, Tian WM (2013) Comparative proteomics of primary and secondary lutoids reveals that chitinase and glucanase play a crucial combined role in rubber particle aggregation in Hevea brasiliensis. J Proteome Res 12:5146–5159

    Article  CAS  PubMed  Google Scholar 

  • Wang X, Wang D, Sun Y, Yang Q, Chang L, Wang L, Meng X, Huang Q, Jin X, Tong Z (2015) Comprehensive proteomics analysis of laticifer latex reveals new insights into ethylene stimulation of natural rubber production. Sci Rep 5:13778

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang D, Sun Y, Tong Z, Yang Q, Chang L, Meng X, Wang L, Tian W, Wang X (2016) A protein extraction method for low protein concentration solutions compatible with the proteomic analysis of rubber particles. Electrophoresis 37:2930–2939

    Article  CAS  PubMed  Google Scholar 

  • Wang D, Xie Q, Sun Y, Tong Z, Chang L, Yu L, Zhang X, Yuan B, He P, Jin X, Dong Y (2019) Proteomic landscape has revealed small rubber particles are crucial rubber biosynthetic machines for ethylene-stimulation in natural rubber production. Int J Mol Sci 20:5082

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wiese S, Reidegeld KA, Meyer HE, Warscheid B (2007) Protein labeling by iTRAQ: a new tool for quantitative mass spectrometry in proteome research. Proteomics 7:340–350

    Article  CAS  PubMed  Google Scholar 

  • Wu X, Xiong E, Wang W, Scali M, Cresti M (2014) Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis. Nat Prot 9:362

    Article  CAS  Google Scholar 

  • Xiang Q, Xia K, Dai L, Kang G, Li Y, Nie Z, Duan C, Zeng R (2012) Proteome analysis of the large and the small rubber particles of Hevea brasiliensis using 2D-DIGE. Plant Physiol Biochem 60:207–213

    Article  CAS  PubMed  Google Scholar 

  • Yagami T, Haishima Y, Tsuchiya T, Tomitaka-Yagami A, Kano H, Matsunaga K (2004) Proteomic analysis of putative latex allergens. Int Arch Allergy Immunol 135:3–11

    Article  CAS  PubMed  Google Scholar 

  • Yamashita S, Yamaguchi H, Waki T, Aoki Y, Mizuno M, Yanbe F, Ishii T, Funaki A, Tozawa Y, Miyagi-Inoue Y, Fushihara K (2016) Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis. elife 5:e19022

    Article  PubMed  PubMed Central  Google Scholar 

  • Yan J, Chen SC (2008a) Preliminary analysis of the proteins expressed differentially of lutoid on the latex of tapping panel dryness (TPD) in rubber tree (Hevea brasiliensis Muell. Arg.). North Hortic 7:58–62

    Google Scholar 

  • Yan J, Chen SC (2008b) Differential analysis of two-dimensional gel electrophoresis profiles and preliminary identification of lutoid proteome on the latex of TPD in Hevea brasiliensis. Hubei Agric Sci 47:858–862

    Google Scholar 

  • Yan J, Chen SC, Xia ZH (2008) Screening and identification of differential expressed proteins in C-serum on latex of tapping panel dryness (TPD) in rubber tree (Hevea brasiliensis Muell. Arg.). China Biotechnol 28:28–36

    Google Scholar 

  • Yeang HY, Arif SAM, Yusof F, Sunderasan E (2002) Allergenic proteins of natural rubber latex. Methods 27:32–45

    Article  CAS  PubMed  Google Scholar 

  • Yuan K, Xu ZJ, Wang ZH, Yang LF (2012) Identification and analysis of latex proteins related to tapping panel dryness in Hevea brasiliensis. J Northwest Univ 27(6):105–109+127

  • Yuan K, Zhou X, Wang Z, Yang L (2014) Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis. J Nanjing For Univ (Natural Sciences Edition) 38:36–40

    CAS  Google Scholar 

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Acknowledgements

This work was supported by the Ministry of Higher Education (MOHE) Malaysia under the Fundamental Research Grant Scheme (FRGS) (203.CABR.6711674). We would like to acknowledge USM Fellowship for supporting the PhD studentship of Mohd Afiq Hazlami Habib.

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Habib, M.A.H., Ismail, M.N. Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward. J Plant Res 134, 43–53 (2021). https://doi.org/10.1007/s10265-020-01231-x

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