Catalytic C–H oxidation is a powerful transformation with enormous promise to streamline access to complex molecules. In recent years, biocatalytic C–H oxidation strategies have received tremendous attention due to their potential to address unmet regio- and stereoselectivity challenges that are often encountered with the use of small-molecule-based catalysts. This Account provides an overview of recent contributions from our laboratory in this area, specifically in the use of iron- and α-ketoglutarate-dependent dioxygenases in chemoenzymatic syntheses of complex natural products. 1 Introduction 2 Overview of Natural Oxygenases 3 C5 Hydroxylation of Aliphatic Amino Acids 4 Chemoenzymatic Synthesis of Tambromycin 5 Chemoenzymatic Synthesis of Cepafungin I and Related Analogues 6 Chemoenzymatic Synthesis of GE81112 B1 and Related Analogues 7 Conclusion and Future Direction

中文翻译：

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铁和α-酮戊二酸依赖性双加氧酶作为天然产物合成中实用生物催化剂的探索

催化 C-H 氧化是一种强大的转化，有望简化复杂分子的获取过程。近年来，生物催化C-H氧化策略因其解决使用小分子催化剂时经常遇到的未满足的区域选择性和立体选择性挑战的潜力而受到极大关注。本报告概述了我们实验室在该领域的最新贡献，特别是在复杂天然产物的化学酶合成中使用铁和α-酮戊二酸依赖性双加氧酶。1 简介 2 天然加氧酶概述 3 脂肪族氨基酸的 C5 羟基化 4 坦溴霉素的化学酶法合成 5 头孢芬净 I 及相关类似物的化学酶法合成 6 GE81112 B1 及相关类似物的化学酶法合成 7 结论和未来方向