In this study, bacterial strains Ha5^T, Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15–37°C (optimum, 28–30°C) and in the presence of 0–1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C_18:1ω7c, C_16:0, C_19:0ω8c cyclo, C_14:0, and C_16:0 2-OH. Strains Ha5^T, Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56–94.63%) with Neokomagataea tanensis NBRC106556^T of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5^T, Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5^T as the type strain.

在这项研究中，Ha5 ^T，Ta1和Jb2细菌菌株是从织叶蚂蚁Oecophylla smaragdina的不同菌落中分离出来的。它们被鉴定为属于醋杆菌科的蚂蚁的细菌共生体，并且基于独特的随机扩增多态性DNA（RAPD）指纹被区分为不同的菌株。这些细菌菌株的细胞为革兰氏阴性，杆状，好氧，不能运动，过氧化氢酶阳性和氧化酶阴性。它们能够在15–37°C（最佳28–30°C）和0–1.5％（w / v）NaCl（最佳0％）的存在下生长。他们的主要细胞脂肪酸为C _18：1个ω 7C，C _16：0，C _19：0 ω 8C环，C_14：0和C _16：0 2-OH。Ha5 ^T，Ta1和Jb2菌株与醋杆菌科新鞭毛藻NBRC106556 ^T具有最高的16S rRNA基因序列相似性（94.56–94.63％）。基于16S rRNA基因序列的系统发育分析和基于核心基因的系统生物学分析都将它们置于醋杆菌科的不同谱系中。这些细菌菌株在多个总体基因组相关性指数中均高于物种水平阈值，这表明它们属于同一物种。此外，它们不属于任何醋杆菌科因为它们与该家族所有类型成员的配对平均核苷酸同一性较低（<71％），计算机DNA-DNA杂交（<38％）和平均氨基酸同一性（<67％）值。基于这些结果，细菌菌株Ha5 ^T，Ta1和Jb2代表醋杆菌科中一个新属的新物种，为此我们建议将其命名为Oecophyllibacter saccharovorans gen。十一月 sp。nov。，并以Ha5 ^T菌株为类型菌株。