The present work was aimed to investigate the phylogenetic relationships of Pentatomidae based on mitochondrial Cyt b gene sequences. We sequenced and analysed a 440 bp fragment of Cyt b gene to assess the level of sequence divergence in different species of Pentatomid bugs collected from distant localities of Northern India. The data divulged an R-value of 1.19 suggesting transitions to be more than transversions. 18 sequences representing 10 species and 5 genera were finally analysed. The intraspecific divergence ranged from 0.2 to 7.2% with an average of 3.75 ± 3.0% whilst interspecific divergence ranged from 3.8 to 10.3% with an average of 7.49 ± 1.7% and intergeneric distance ranged from 12.1 to 20.2% with an average of 16.37 ± 2.2%. The percentage of divergence values revealed a clear pattern of increased nucleotide diversity from conspecific to congeneric to intra subfamily level using Cyt b gene fragment. Phylogenetic trees were constructed using Neighbor-Joining method that revealed the phylogenetic status of Indian Pentatomid bugs with other species from different geographical locations. The results were also confirmed with additional molecular parameter, nucleotide substitution and pairwise genetic distance.

中文翻译：

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五翅目不同种间细胞色素b序列差异及系统发育关系（半翅目：异翅目）

目前的工作旨在基于线粒体Cyt b基因序列研究五足科的系统发育关系。我们对 Cyt b 基因的 440 bp 片段进行了测序和分析，以评估从印度北部偏远地区收集的不同种类的 Pentatomid bug 的序列差异水平。数据透露的 R 值为 1.19，表明转换不仅仅是颠换。最终分析了代表10种5属的18个序列。种内差异范围为 0.2 至 7.2%，平均为 3.75±3.0%，而种间差异范围为 3.8 至 10.3%，平均为 7.49±1.7%，属间距离范围为 12.1 至 20.2%，平均为 7±12.26。 %。差异值的百分比揭示了使用 Cyt b 基因片段从同种到同种再到亚科内水平的核苷酸多样性增加的清晰模式。系统发育树是使用 Neighbor-Joining 方法构建的，该方法揭示了 Indian Pentatomid bug 与来自不同地理位置的其他物种的系统发育状态。结果还通过额外的分子参数、核苷酸取代和成对遗传距离得到证实。