Abstract
The present work was aimed to investigate the phylogenetic relationships of Pentatomidae based on mitochondrial Cyt b gene sequences. We sequenced and analysed a 440 bp fragment of Cyt b gene to assess the level of sequence divergence in different species of Pentatomid bugs collected from distant localities of Northern India. The data divulged an R-value of 1.19 suggesting transitions to be more than transversions. 18 sequences representing 10 species and 5 genera were finally analysed. The intraspecific divergence ranged from 0.2 to 7.2% with an average of 3.75 ± 3.0% whilst interspecific divergence ranged from 3.8 to 10.3% with an average of 7.49 ± 1.7% and intergeneric distance ranged from 12.1 to 20.2% with an average of 16.37 ± 2.2%. The percentage of divergence values revealed a clear pattern of increased nucleotide diversity from conspecific to congeneric to intra subfamily level using Cyt b gene fragment. Phylogenetic trees were constructed using Neighbor-Joining method that revealed the phylogenetic status of Indian Pentatomid bugs with other species from different geographical locations. The results were also confirmed with additional molecular parameter, nucleotide substitution and pairwise genetic distance.
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Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389–3402
Avise JC (1994) Molecular markers, natural history and evolution. Chapman and Hall, New York, p 511
Blair JE, Hedges SB (2005) Molecular phylogeny and divergence times of Deuterostome animals. Mol Biol Evol 22(11):2275–2284
Blair CP, Abrahamson WG, Jackman JA, Tyrrell I (2005) Cryptic speciation and host race formation in a purportedly generalist tumbling flower beetle. Evolution 59:304–316
Boore JL (1999) Animal mitochondrial genomes. Nucleic Acids Res 27(8):1767–1780
Brown WM, George M, Wilson AC (1979) Rapid evolution of animal mitochondrial DNA. Proc Natl Acad Sci U S A 76(4):1967–1971
Cameron SL, Lambkin CL, Barker SC, Whiting MF (2007) A mitochondrial genome phylogeny of Diptera: whole genome sequence data accurately resolve relationships over broad timescales with high precision. Syst Entomol 32(1):40–59
Dolling WR (1981) A rationalized classification of the burrower bugs (Cydnidae). Syst Entomol 6:61–76
Dowton M, Austin AD, Antolin MF (1998) Evolutionary relationships among the Braconidae (Hymenoptera: Ichneumonoidea) inferred from partial 16S rDNA gene sequences. Insect Mol Biol 7(2):129–150
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32(5):1792–1797
Galtier N, Enard D, Radondy Y, Bazin E (2005) Mutation hot spots in mammalian mitochondrial DNA. Genome Res 16:215–222
Goetze E (2003) Cryptic speciation on the high seas; global phylogenetics of the copepod family Eucalanidae. Proc R Soc Lond 270:2321–2331
Hendrixson BE, Bond JE (2005) Testing species boundaries in the Anthrodiaetus unicolor complex (Araneae: Mygalomorphae: Antrodiaetidae): Paraphyly and cryptic diversity. Mol Phylogenet Evol 36:405–416
Huelsenbeck JP, Ronquist F, Nielsen R, Bollback JP (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294(5550):2310–2314
Jinbo U, Kato T, Ito M (2011) Current progress in DNA barcoding and future implications for entomology. Entomol Sci 14(2):107–124
Kambhampati S, Rai KS (1991) Mitochondrial DNA variation within and among populations of the mosquito Aedes albopictus. Genome 34(2):288–292
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kumar S, Tamura K, Nei M (1994) MEGA: molecular evolutionary genetics analysis software for microcomputers. Bioinformatics 10(2):189–191
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17(12):1244–1245
Lessinger AC, Junqueira ACM, Lemos TA, Kemper EL, da Silva FR, Vettore AL, Arruda P, Azeredo-Espin AML (2000) The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae). Insect Mol Biol 9:521–529
Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods Enzymol 155:335–350
Muraji M, Kenjiro K, Toru S (2000) Phylogenetic utility of nucleotide sequences of mitochondrial 16S ribosomal RNA and cytochrome b genes in anthocorid bugs (Heteroptera: Anthocordiae). Appl Entomol Zool 35(3):293–300
Park DS, Foottit R, Maw E, Hebert PD (2011) Barcoding bugs: DNA based identification of the true bugs (Insecta: Hemiptera: Heteroptera). PLoS One 6(4):1–9
Regier JC, Shultz JW, Kambic RE (2005) Pancrustacean phylogeny: hexapods are terrestrial crustaceans and maxillopods are not monophyletic. Proc R Soc London Ser B 272:395–401
Rider DA (2019) https://www.ndsu.nodak.edu/ndsu/rider/pentatomoidea/. Accessed 27 June 2019
Roe AD, Sperling FAH (2007) Patterns of evolution of mitochondrial cytochrome c oxidase I and II DNA and implications for DNA barcoding. Mol Phylogenet Evol 44:325–345
Rubinoff D, Holland BS (2005) Between two extremes: mitochondrial DNA is neither the panacea nor the nemesis of phylogenetic and taxonomic inference. Syst Biol 54:92–961
Safavi M (1973) Cle dé détermination des families d’Hémiptéres Hétérotéres de l’Iran. Journal of Entomological Society of Iran 1(1):3–11
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci 74(12):5463–5467
Schaefer CW, Panizzi AR (2000) Heteroptera of economic importance. CRC Press, Boca Raton
Schuh RT, Slater JA (1995) True bugs of the world (Hemiptera: Heteroptera) classification and natural history. Cornell University Press, Ithaca
Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701
Song N, Liang A (2009) The complete mitochondrial genome sequence of Geisha distinctissima (Hemiptera: Flatidae) and comparison with other hemipteran insects. Acta Biochim Biophys Sin 41(3):206–216
Souza HV, Marchesin SRC, Itoyama MM (2016) Analysis of the mitochondrial COI gene and its informative potential for evolutionary inferences in the families Coreidae and Pentatomidae (Heteroptera). Genet Mol Res 15(1):1–14
Sperling FAH, Hickey DA (1994) Mitochondrial DNA sequence variation in the spruce budworm species complex (Choristoneura: Lepidoptera). Mol Biol Evol 11:656–665
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Tian X, Xie Q, Li M, Gao C, Cui Y, Xi L, Bu W (2011) Phylogeny of pentatomomorphan bugs (Hemiptera-Heteroptera: Pentatomomorpha) based on six Hox gene fragments. Zootaxa 2888(1):57–68
Wang Y, Chen J, Jiang LY, Qiao GX (2015) Hemipteran mitochondrial genomes: features, structures and implications for phylogeny. Int J Mol Sci 16(6):12382–12404
Weirauch C, Schuh RT (2011) Systematics and evolution of Heteroptera: 25 years of progress. Annu Rev Entomol 56:487–510
Acknowledgments
We acknowledge Rajiv Gandhi National Fellowship for financial support and DBT-IPLS Project for required laboratory facilities at Sophisticated Instrumentation Centre. This study was conducted as part of a doctoral research by the first author, R.K. at Zoology Department, Punjabi University, Patiala.
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Kaur, R., Singh, D. Cytochrome b sequence divergence and phylogenetic relationships among different species of family Pentatomidae (Hemiptera: Heteroptera). Int J Trop Insect Sci 41, 1177–1183 (2021). https://doi.org/10.1007/s42690-020-00303-8
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DOI: https://doi.org/10.1007/s42690-020-00303-8