Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.

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细胞内显微操作的高级生物成像：方法和应用

机器人系统辅助的细胞内显微操作在生物医学研究中具有重要的应用，例如基因诊断和基因组编辑任务。但是，由于针对标本的手术信息的信息不足，因此目前的研究成功率低，手术损害大。细胞内环境的复杂性导致难以可视化操作工具和标本。这篇综述总结并分析了细胞内显微操作应用中高级生物成像采样和计算处理方法的最新发展。它还讨论了相关的限制和将来的扩展，为该领域提供了重要的参考。