Abstract

This study deals with participation of isoquinoline derivatives of coumarin in the peroxidase reaction catalyzed by the cytochrome c–cardiolipin complex. We have studied coumarin derivatives called coumarin-314 (C-314), coumarin-334 (C-334) and coumarin-525 (C-525). These substances are considered as specific physical activators of chemiluminescence, which accompanies the lipid peroxidation reaction. In the course of the scientific research, a spectrophotometric study of the effect of methanol on the structure of cytochrome c was performed, the optical properties of these substances were studied in the medium of a phosphate buffer, and a spectrophotometric study was performed with parallel registration of the chemiluminescence of a mixture in which cytochrome c catalyzed by a complex with cardiolipin lipoperoxidase reaction in the presence of isoquinolysin derivatives of coumarin. The conclusions of this work contain identification of the reversibility of the action of methanol on the structure of cytochrome c, which proves the possibility of using this alcohol in the study of this protein, the positions of absorption maxima and corresponding values of molar absorption coefficients in the medium of 20 mM phosphate buffer (pH = 7.4) for C-314 (λ_max = 447.5 nm; ε = 32360.4 L/(mol cm)), C-334 (λ_max = 460 nm; ε = 44 012 L/(mol cm)), and C-525 (λ_max = 460 nm; ε = 32 703.56 L/(mol cm)). We also demonstrated that the said substances are substrates of cytochrome c/cardiolipin complex-catalyzed peroxidase reaction. Mass flow of these substances during an average statistical experiment in measurement of chemiluminescence (322 s) for C-314, C-334 and C-525 amounted to 31.89, 37.61 and 25.94%, respectively.

中文翻译：

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异喹啉香豆素衍生物作为脂质过氧化反应中的化学发光活化剂

摘要

这项研究涉及香豆素的异喹啉衍生物参与细胞色素c催化的过氧化物酶反应–心磷脂复合物。我们研究了称为香豆素314（C-314），香豆素334（C-334）和香豆素525（C-525）的香豆素衍生物。这些物质被认为是化学发光的特定物理活化剂，它伴随脂质过氧化反应。在科学研究过程中，进行了分光光度法研究甲醇对细胞色素c结构的影响，在磷酸盐缓冲液的介质中研究了这些物质的光学性质，并通过平行配准进行了分光光度法研究。香豆素异喹啉溶素衍生物存在下由心磷脂脂过氧化物酶反应复合物催化细胞色素c的混合物的化学发光c，这证明了在这种蛋白质的研究中使用这种醇的可能性，C-314的_最大吸收位置以及在20 mM磷酸盐缓冲液（pH = 7.4）中的摩尔吸收系数的相应值（_λmax = 447.5 nm;ε= 32360.4 L /（mol cm）），C-334（_λmax = 460 nm;ε= 44012 L /（mol cm））和C-525（_λmax = 460 nm;ε= 32 703.56 L /（mol cm））。我们还证明了所述物质是细胞色素c /心磷脂复合物催化的过氧化物酶反应的底物。在测量C-314，C-334和C-525的化学发光（322 s）的平均统计实验期间，这些物质的质量流量分别为31.89％，37.61％和25.94％。