Ratiometric persistent luminescence aptasensors for carcinoembryonic antigen detection,Microchimica Acta

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Ratiometric persistent luminescence aptasensors for carcinoembryonic antigen detection
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-10-19 , DOI: 10.1007/s00604-020-04593-0
Lixia Shi ₁ , Wangwang Zheng ₁ , Hongyan Miao ₁ , Han Liu ₁ , Xiaohui Jing ₁ , Yuan Zhao ₁

Affiliation

NIR-emitted ZnGa2O4:Cr3+ persistent luminescence nanoparticles (ZGC NPs) coated with polydopamine (ZGC@PDA NPs) were designed featuring internal reference and quenching ability. Sr-doped Zn2GeO4 persistent luminescence nanorods (ZGO:Sr NRs) served as detection probes, which exhibited blue emission. The decay times and intensity of luminescence of ZGO:Sr NRs were optimized to acquire desired luminescence properties. An aptamer-guided ratiometric persistent luminescence sensor with the LOD (0.46 pg mL−1) was established to detect carcinoembryonic antigen (CEA). This developed ratiometric aptasensor based on persistent luminescence nanomaterials (PLMs) does not only use the afterglow properties of nanomaterials to avoid the interference of autofluorescence but also precludes the interference of certain factors in the detection environment on the luminescence intensity due to the introduction of a reference signal, and is suitable for early screening of tumor markers in serum samples. Moreover, the optimization of luminescence properties, especially for luminescence decay times, provides a way for the fabrication of multiple persistent luminescence materials in the application of time-resolved fluorescence technology. Graphical abstract Construction of ZGO:Sr NR- and ZGC@PDA NP-driven ratiometric aptasensor for CEA detection. Construction of ZGO:Sr NR- and ZGC@PDA NP-driven ratiometric aptasensor for CEA detection.

中文翻译：

用于癌胚抗原检测的比率持续发光适体传感器

设计了涂有聚多巴胺 (ZGC@PDA NPs) 的近红外发射 ZnGa2O4:Cr3+ 持久发光纳米粒子 (ZGC NPs)，具有内参和淬灭能力。掺杂 Sr 的 Zn2GeO4 持久发光纳米棒 (ZGO:Sr NRs) 作为检测探针，呈现蓝色发射。ZGO:Sr NRs 的衰减时间和发光强度经过优化以获得所需的发光特性。建立了一种具有 LOD (0.46 pg mL-1) 的适体引导的比率持续发光传感器来检测癌胚抗原 (CEA)。这种基于持久发光纳米材料（PLMs）开发的比率适体传感器不仅利用纳米材料的余辉特性来避免自发荧光的干扰，而且由于引入了参考，排除了检测环境中某些因素对发光强度的干扰。信号，适用于血清样本中肿瘤标志物的早期筛查。此外，发光特性的优化，特别是发光衰减时间的优化，为时间分辨荧光技术应用中多种持久发光材料的制备提供了途径。ZGO:Sr NR 和 ZGC@PDA NP 驱动的用于 CEA 检测的比率适体传感器的图形摘要构建。ZGO的建设：

更新日期：2020-10-19

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