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Ratiometric persistent luminescence aptasensors for carcinoembryonic antigen detection

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Abstract

NIR-emitted ZnGa2O4:Cr3+ persistent luminescence nanoparticles (ZGC NPs) coated with polydopamine (ZGC@PDA NPs) were designed featuring internal reference and quenching ability. Sr-doped Zn2GeO4 persistent luminescence nanorods (ZGO:Sr NRs) served as detection probes, which exhibited blue emission. The decay times and intensity of luminescence of ZGO:Sr NRs were optimized to acquire desired luminescence properties. An aptamer-guided ratiometric persistent luminescence sensor with the LOD (0.46 pg mL−1) was established to detect carcinoembryonic antigen (CEA). This developed ratiometric aptasensor based on persistent luminescence nanomaterials (PLMs) does not only use the afterglow properties of nanomaterials to avoid the interference of autofluorescence but also precludes the interference of certain factors in the detection environment on the luminescence intensity due to the introduction of a reference signal, and is suitable for early screening of tumor markers in serum samples. Moreover, the optimization of luminescence properties, especially for luminescence decay times, provides a way for the fabrication of multiple persistent luminescence materials in the application of time-resolved fluorescence technology.

Construction of ZGO:Sr NR- and ZGC@PDA NP-driven ratiometric aptasensor for CEA detection.

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Acknowledgments

The authors thank the Central Laboratory, School of Chemistry and Materials Engineering, Jiangnan University, for technical support.

Funding

The authors acknowledged the financial support by the Natural Science Foundation of Jiangsu Province (BK20171136), National Key Research and Development Program of China (2017YFC1601706), and National First-class Discipline Program of Food Science and Technology (JUFSTR20180302).

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Authors and Affiliations

  1. Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, International Joint Research Center for Photoresponsive Molecules and Materials, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Lixia Shi, Wangwang Zheng, Hongyan Miao, Han Liu, Xiaohui Jing & Yuan Zhao

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  1. Lixia Shi
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  2. Wangwang Zheng
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  3. Hongyan Miao
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  4. Han Liu
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  5. Xiaohui Jing
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  6. Yuan Zhao
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Correspondence to Yuan Zhao.

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Shi, L., Zheng, W., Miao, H. et al. Ratiometric persistent luminescence aptasensors for carcinoembryonic antigen detection. Microchim Acta 187, 615 (2020). https://doi.org/10.1007/s00604-020-04593-0

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