Herein, we studied the effect of labile iron (ferric chloride) on the progression of liver cancer cells (HepG2.2.15). The iron was found to induce cell proliferation, growth, and migration in both traditional two-dimensional (2D) and three-dimensional cell (3D) culture models. Biophysical and cell cycle determinations also showed the change in functional cellular biophysical features (cell morphology) and cell cycle kinetic during cancer cell growth induced by the labile iron. According to immunofluorescence and the iron uptake inhibition studies, L-type calcium channel was found to plays a role in the iron uptake in the liver cancer cells. This report gives new insights into iron-mediated cancer cell growth and will pave the new way to diagnosis and treatment of liver cancer.

在这里，我们研究了不稳定的铁（氯化铁）对肝癌细胞（HepG2.2.15）进程的影响。发现铁在传统的二维（2D）和三维细胞（3D）培养模型中均诱导细胞增殖，生长和迁移。生物物理和细胞周期测定还显示了不稳定铁诱导的癌细胞生长过程中功能性细胞生物物理特征（细胞形态）和细胞周期动力学的变化。根据免疫荧光和铁摄取抑制研究，发现L型钙通道在肝癌细胞中的铁摄取中起作用。该报告为铁介导的癌细胞生长提供了新见解，并将为肝癌的诊断和治疗铺平道路。