Ninety five bacterial strains were isolated from the rhizosphere and soils of flower crops, specifically gerbera, carnation and tuberose, were preliminarily screened against Fusarium oxysporum f. sp. gerberae in vitro along with five elite strains Bbv 57, EPCO 16, EPC 5, EPC 8 and Pf 1 obtained from Department of Plant Pathology, TNAU, Coimbatore, India. The results revealed that the strain Bbv 57 had highest inhibition of 50.00% and showed the maximum value for the assays that is siderophore, hydrogen cyanide, IAA, GA3 and salicylic acid production (3.68 O.D. value; 14.05 µg ml^-1; 44.40 µg ml^-1; 25.28 µg ml^-1 and 19.25 µg ml^-1 respectively). It also showed resistance to antibiotics namely ampicillin, erythromycin, clindamycin. The highest exopolysaccharides, biofilm production was observed and had lowest protease production clearly indicated that it is non - pathogenic to plants. Further, the polymerase chain reaction using16S rRNA intervening sequencing showed 100% homology to Bacillus subtilis (KF718836) and showed positive for quorum sensing regulator genes of aiiA and comQ. Additionally, the culture filtrate assay also produced significant reduction in egg hatching capacity and juvenile mortality of root-knot nematode Meloidogyne incognita. Further, the HPLC study showed 91.69 µg µl^-1 of surfactin with the retention time of 2.304 min and 0.453 µg µl^-1 of Iturin with the retention time of 8.739 min at 205 nm. Whereas, GCMS analysis has detected the aliphatic hydrocarbons responsible for antifungal, antibacterial and nematicidal activity.

九个十五细菌菌株从根际和花卉作物，特别非洲菊，香石竹和晚香玉的土壤中分离，进行了初步筛选针对尖孢镰刀菌F。sp。非洲菊非洲菊和五种从印度Coimbatore TNAU的植物病理学系获得的优良菌株Bbv 57，EPCO 16，EPC 5，EPC 8和Pf 1。结果显示，菌株Bbv 57的抑制率最高，为50.00％，并显示出铁载体，氰化氢，IAA，GA3和水杨酸生成的最大值（OD值3.68； 14.05 µg ml ^-1； 44.40 µg ml ^-1 ; 25.28 µg ml ^-1和19.25 µg ml ^-1分别）。它还显示出对氨苄青霉素，红霉素，克林霉素等抗生素的抗药性。观察到最高的胞外多糖，生物膜产量，而最低的蛋白酶产量清楚地表明它对植物无致病性。此外，使用16S rRNA插入测序的聚合酶链反应显示出与枯草芽孢杆菌（KF718836）的100％同源性，并显示aiiA和comQ的群体感应调节基因阳性。另外，培养物滤液测定法还导致卵根结线虫Meloidogyne incognita的卵孵化能力和幼虫死亡率显着降低。此外，HPLC研究显示91.69 µg µl ^-1表面活性素的保留时间为2.304分钟，而Iturin的保留时间为0.453 µg µl ^-1，在205 nm处的保留时间为8.739分钟。相比之下，GCMS分析已检测到负责抗真菌，抗菌和杀线虫活性的脂族烃。