Breast cancer (BC) is a major concern to female health worldwide. We assessed the circulating osteocyte‐related biomarkers, hepcidin, and oxidative stress status among early-stage BC patients in aspects of clinical severity and impact on the outcome. The study incorporated 73 patients categorized into 57 early-stage BC and 16 benign breast diseases and 30 healthy controls. Serum 25-hydroxyvitamin D [25(OH)D], sclerostin (SOST), dickkopf-1(DKK1), and hepcidin were measured using ELISA, while, serum oxidative stress markers were assessed by spectrophotometry. Our results show that patients with BC showed significant increase in the mean levels of DKK1, SOST, hepcidin, and LPER and significant decrease in the mean levels of 25(OH)D, SOD, GPx, and Hb when compared with controls and benign breast diseases. Significantly higher DKK1, hepcidin, and SOD levels among benign breast diseases were found in comparison to control group. There were significantly lower levels of 25(OH)D, SOD, and Hb and significantly higher levels of SOST, DKK1, hepcidin, No, and LPER with advanced grade. Lower levels of 25(OH)D, SOD and higher levels of SOST, hepcidin were observed with increasing the malignant stage. Reduced levels of 25(OH)D, and SOD were significantly associated with poor prognosis and were strong predictors among BC. There were significant negative correlations between 25(OH)D with LPER, SOST, and hepicidin. We conclude that low 25(OH)D, high SOST, DKK1, and hepcidin, and dysregulated oxidative stress could be helpful in early detection and assessment of BC. 25(OH)D, and SOD were the most relevant to tumor progression and prognosis which indicate a significant role in the BC pathogenesis and could be promising targets in management. Our research paves the way to disrupt vicious circle between these biomarkers to obtain the best care of BC.

乳腺癌（BC）是全世界女性健康的主要关注点。我们从临床严重程度和对预后的影响方面评估了早期BC患者中循环的骨细胞相关生物标志物，铁调素和氧化应激状态。该研究纳入了73位患者，这些患者被归类为57例早期BC，16例良性乳腺疾病和30例健康对照。用ELISA法测定血清25-羟基维生素D [25（OH）D]，硬化素（SOST），dickkopf-1（DKK1）和铁调素，同时通过分光光度法评估血清氧化应激标志物。我们的结果表明，与对照组和良性乳腺相比，BC患者的DKK1，SOST，铁调素和LPER的平均水平显着升高，而25（OH）D，SOD，GPx和Hb的平均水平显着降低。疾病。DKK1，hepcidin，与对照组相比，乳腺良性疾病的SOD和SOD水平更高。具有较高等级的25（OH）D，SOD和Hb的水平明显较低，而SOST，DKK1，hepcidin，No和LPER的水平则显着较高。随着恶性程度的增加，观察到较低水平的25（OH）D，SOD和较高水平的SOST，铁调素。25（OH）D和SOD水平的降低与不良预后显着相关，并且是BC的强预测指标。25（OH）D与LPER，SOST和hepicidin之间存在显着的负相关。我们得出的结论是，低25（OH）D，高SOST，DKK1和铁调素以及氧化应激失调可能有助于早期发现和评估BC。25（OH）D SOD和SOD与肿瘤的进展和预后最相关，这表明在BC发病机理中具有重要作用，并且可能是有希望的治疗靶标。我们的研究为破坏这些生物标记之间的恶性循环铺平了道路，从而获得了最佳的BC护理。