Abstract

In order to obtain hydrogel matrices for bone tissue engineering, the process of formation of highly filled alginate-calcium phosphate structures by 3D printing was developed. Optimal conditions for the formation of three-dimensional structures from Ca²⁺ crosslinked alginate hydrogels and highly filled alginate compositions with fine (5–30 μm) α-tricalcium phosphate (TCP) were determined. Comparative analysis of physicomechanical properties of crosslinked alginate hydrogels showed lower strength and higher elastic modulus of the TCP-filled composite in comparison with pure hydrogel. The difference between the mechanical characteristics of the filled and pure gels increases with the crosslinking density. It was found that, because of the significant content of the mineral dispersed phase, the α-TCP-filled hydrogel does not shrink during crosslinking, unlike pure alginate hydrogel. Using cultures of human umbilical cord mesenchymal stem cells, it was shown in vitro that all the studied samples of both pure and highly filled composite alginate matrices with α-TCP do not have short-term cytotoxic effects.

中文翻译：

---

基于藻酸盐凝胶和精细磷酸三钙的高填充组合物，用于组织工程基质的3D打印

摘要

为了获得用于骨组织工程的水凝胶基质，开发了通过3D打印形成高度填充的藻酸盐-磷酸钙结构的过程。Ca ²⁺形成三维结构的最佳条件确定了交联的藻酸盐水凝胶和高度填充的藻酸盐成分，这些成分具有细（5–30μm）的α-磷酸三钙（TCP）。交联海藻酸盐水凝胶的物理力学性能的比较分析表明，与纯水凝胶相比，TCP填充复合材料的强度和弹性模量较低。填充凝胶和纯凝胶的机械特性之间的差异随交联密度的增加而增加。已经发现，由于矿物分散相的含量高，与纯藻酸盐水凝胶不同，填充α-TCP的水凝胶在交联期间不会收缩。使用人脐带间充质干细胞培养