Skip to main content
SpringerLink
Log in

Highly Filled Compositions Based on Alginate Gel and Fine Tricalcium Phosphate for 3D Printing of Tissue-Engineered Matrices

  • MATERIALS FOR ENSURING HUMAN VITAL ACTIVITY AND ENVIRONMENTAL PROTECTION
  • Published:
Inorganic Materials: Applied Research Aims and scope

Abstract

In order to obtain hydrogel matrices for bone tissue engineering, the process of formation of highly filled alginate-calcium phosphate structures by 3D printing was developed. Optimal conditions for the formation of three-dimensional structures from Ca2+ crosslinked alginate hydrogels and highly filled alginate compositions with fine (5–30 μm) α-tricalcium phosphate (TCP) were determined. Comparative analysis of physicomechanical properties of crosslinked alginate hydrogels showed lower strength and higher elastic modulus of the TCP-filled composite in comparison with pure hydrogel. The difference between the mechanical characteristics of the filled and pure gels increases with the crosslinking density. It was found that, because of the significant content of the mineral dispersed phase, the α-TCP-filled hydrogel does not shrink during crosslinking, unlike pure alginate hydrogel. Using cultures of human umbilical cord mesenchymal stem cells, it was shown in vitro that all the studied samples of both pure and highly filled composite alginate matrices with α-TCP do not have short-term cytotoxic effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Fig. 7.

Similar content being viewed by others

REFERENCES

  1. Griffith, L.G. and Naughton, G., Tissue engineering—current challenges and expanding opportunities, Science, 2002, vol. 295, no. 5557, pp. 1009–1014.

    Article  CAS  Google Scholar 

  2. Sevast’yanov, V.I. and Kirpichnikov, M.P., Biosovmestimye materialy (Biocompatible Materials), Moscow: Med. Inf. Agentstvo, 2011.

  3. Atala, A., Engineering organs, Curr. Opin. Biotechnol., 2009, vol. 20, no. 5, pp. 575–592.

    Article  CAS  Google Scholar 

  4. Hutmacher, D.W., Goh, J.C., and Teoh, S.H., An introduction to biodegradable materials for tissue engineering applications, Ann. Acad. Med. Singapore, 2001, vol. 30, no. 2, pp. 183–191.

    CAS  PubMed  Google Scholar 

  5. Fisher, J.P. and Reddi, A.H., Functional tissue engineering of bone: signals and scaffolds, Topics Tissue Eng., 2003, vol. 1, pp. 1–29.

    Google Scholar 

  6. Zhu, J. and Marchant, R.E., Design properties of hydrogel tissue-engineering scaffolds, Expert Rev. Med. Devices, 2011, vol. 8, pp. 607–626.

    Article  Google Scholar 

  7. Ahmed, E.M., Hydrogel: preparation, characterization, and applications: a review, J. Adv. Res., 2015, vol. 6, no. 2, pp. 105–121.

    Article  CAS  Google Scholar 

  8. El-Sherbiny, I.M. and Yacoub, M.H., Hydrogel scaffolds for tissue engineering: progress and challenges, Global Cardiol. Sci. Pract., 2013, vol. 2013, no. 3, pp. 317–342.

    Google Scholar 

  9. Josef, E., Zilberman, M., and Bianco-Peled, H., Composite alginate hydrogels: an innovative approach for the controlled release of hydrophobic drugs, Acta Biomater., 2010, vol. 6, no. 12, pp. 4642–4649.

    Article  CAS  Google Scholar 

  10. Webber, R.E. and Shull, K.R., Strain dependence of the viscoelastic properties of alginate hydrogels, Macromolecules, 2004, vol. 37, no. 16, pp. 6153–6160.

    Article  CAS  Google Scholar 

  11. Sarker, B., Singh, R., Silva, R., Roether, J.A., Kaschta, J., Detsch, R., Schubert, D.W., Cicha, I., and Boccaccini, A.R., Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel, PLoS One, 2014, vol. 9, no. 9, p. e107952.

    Article  Google Scholar 

  12. Contessi Negrini, N., Bonnetier, M., Giatsidis, G., Orgill, D.P., Faré, S., and Marelli, B., Tissue-mimicking gelatin scaffolds by alginate sacrificial templates for adipose tissue engineering, Acta Biomater., 2019, vol. 87, pp. 61–75.

    Article  CAS  Google Scholar 

  13. You, F., Wu, X., and Chen, X., 3D printing of porous alginate/gelatin hydrogel scaffolds and their mechanical property characterization, Int. J. Polym. Mater. Polym. Biomater., 2017, vol. 66, no. 6, pp. 299–306.

    Article  CAS  Google Scholar 

  14. Gnanaprakasam Thankam, F. and Muthu, J., Alginate based hybrid copolymer hydrogels—influence of pore morphology on cell–material interaction, Carbohydr. Polym., 2014, vol. 112, pp. 235–244.

    Article  CAS  Google Scholar 

  15. Kühn, P.T., Rozenbaum, R.T., Perrels, E., Sharma, P.K., and van Rijn, P., Anti-microbial biopolymer hydrogel scaffolds for stem cell encapsulation, Polymers, 2017, vol. 9, no. 4, pp. 149–158.

    Article  Google Scholar 

  16. Luo, Y., Lode, A., Akkineni, A.R., and Gelinsky, M., Concentrated gelatin/alginate composites for fabrication of pre-designed scaffolds with a favorable cell response by 3D plotting, RSC Adv., 2015, vol. 5, no. 54, pp. 43480–4348.

    Article  CAS  Google Scholar 

  17. Liu, Q., Li, Q., Xu, S., Zheng, Q., and Cao, X., Preparation and properties of 3D printed alginate–chitosan polyion complex hydrogels for tissue engineering, Polymers, 2018, vol. 10, no. 6, pp. 664–677.

    Article  Google Scholar 

  18. Giannitelli, S., Mozetic, P., Trombetta, M., and Rainer, A., Additive manufacturing of pluronic/alginate composite thermogels for drug and cell delivery, in Additive Manufacturing, Boca Raton, FL: CRC Press, 2015, pp. 403–412.

    Google Scholar 

  19. Izadifar, Z., Chang, T., Kulyk, W., Chen, X., and Eames, B.F., Analyzing biological performance of 3D-printed, cell-impregnated hybrid constructs for cartilage tissue engineering, Tissue Eng.,Part C, 2016, vol. 22, no. 3, pp. 173–188.

    Article  CAS  Google Scholar 

  20. Bendtsen, S.T., Quinnell, S.P., and Wei, M., Development of a novel alginate-polyvinyl alcohol-hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds, J. Biomed. Mater. Res., Part A, 2017, vol. 105, no. 5, pp. 1457–1468.

    Article  CAS  Google Scholar 

  21. Barradas, A.M.C., Yuan, H., van Blitterswijk, C.A., and Habibovic, P., Osteoinductive biomaterials: Current knowledge of properties, experimental models and biological mechanisms, Eur. Cells Mater., 2011, vol. 21, pp. 407–429.

    Article  CAS  Google Scholar 

  22. Urruela-Barrios, R., Ramírez-Cedillo, E., de León, A.D., Alvarez, A.J., and Ortega-Lara, W., Alginate/gelatin hydrogels reinforced with TiO2 and β-TCP fabricated by microextrusion-based printing for tissue regeneration, Polymers, 2019, vol. 11, no. 3, pp. 457–471.

    Article  Google Scholar 

  23. Koski, C., Onuike, B., Bandyopadhyay, A., and Bose, S., Starch-hydroxyapatite composite bone scaffold fabrication utilizing a slurry extrusion-based solid freeform fabricator, Addit. Manuf., 2018, vol. 24, pp. 47–59.

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Zobkov, Y.V., Mironov, A.V., Fedotov, A.Y., Popov, V.K., Smirnov, I.V., Barinov, S.M., and Komlev V.S., In situ formation of porous mineral–polymer scaffold for tissue engineering, Dokl. Chem., 2017, vol. 474, no. 1, pp. 126–128.

    Article  CAS  Google Scholar 

  25. Maiti, B. and Díaz Díaz, D., 3D printed polymeric hydrogels for nerve regeneration, Polymers, 2018, vol. 10, no. 9, pp. 1041–1053.

    Article  Google Scholar 

  26. Yanagawa, F., Sugiura, S., and Kanamori, T., Hydrogel microfabrication technology toward three dimensional tissue engineering, Regener. Ther., 2016, vol. 3, pp. 45–57.

    Article  Google Scholar 

  27. Chen, J.-P. and Cheng, T.-H., Bone regeneration using 3D hyaluronic acid and chitosan-containing thermoreversible hydrogel and canine mesenchymal stem cells, J. Biotechnol., 2008, vol. 136, pp. 128–129.

    Article  Google Scholar 

  28. Jose, R.R., Rodriguez, M.J., Dixon, T.A., Omenetto, F., and Kaplan, D.L., Evolution of bioinks and additive manufacturing technologies for 3D bioprinting, ACS Biomater. Sci. Eng., 2016, vol. 2 (10, pp. 1662–1678.

  29. Fedotov, A.Y., Egorov, A.A., Zobkov, Y.V., Mironov, A.V., Popov, V.K., Barinov, S.M., and Komlev, V.S., 3D printing of mineral-polymer structures based on calcium phosphate and polysaccharides for tissue engineering, Inorg. Mater.: Appl. Res., 2016, vol. 7, no. 2, pp. 240–243.

    Article  Google Scholar 

  30. Wilson, A.P., Cytotoxicity and viability assays, in Animal Cell Culture, Oxford: Oxford Univ. Press, 2000, vol. 1, pp. 175–219.

    Google Scholar 

Download references

Funding

This work was supported by the Ministry of Science and Higher Education of Russia in the framework of the work on the State Assignment of the Federal Research Center Crystallography and Photonics of the Russian Academy of Sciences regarding the development of 3D printing processes for hydrogel matrices and the Russian Foundation for Basic Research (project no. 18-29-11081_mk) regarding the fabrication and investigation of compositions based on sodium alginate and fine tricalcium phosphate.

Author information

Authors and Affiliations

  1. Federal Research Center Crystallography and Photonics, Russian Academy of Sciences, 119333, Moscow, Russia

    A. V. Mironov, O. A. Mironova, A. O. Mariyanats & V. K. Popov

  2. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, 119334, Moscow, Russia

    V. S. Komlev & I. V. Smirnov

  3. Research Institute of Human Morphology, 117418, Moscow, Russia

    E. Y. Kananykhina & T. Kh. Fatkhudinov

Authors
  1. A. V. Mironov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. A. Mironova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. O. Mariyanats
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. S. Komlev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. V. Smirnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. Y. Kananykhina
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. Kh. Fatkhudinov
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. V. K. Popov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to A. V. Mironov, V. S. Komlev or E. Y. Kananykhina.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by K. Lazarev

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mironov, A.V., Mironova, O.A., Mariyanats, A.O. et al. Highly Filled Compositions Based on Alginate Gel and Fine Tricalcium Phosphate for 3D Printing of Tissue-Engineered Matrices. Inorg. Mater. Appl. Res. 11, 1137–1143 (2020). https://doi.org/10.1134/S2075113320050214

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2075113320050214

Keywords:

Search

Navigation