Pseudomonas syringae pv. actinidiae (Psa) has caused severe damage to kiwifruit plantations worldwide. It is still crucial to find compounds that are able to control the disease. In this work we developed a time and space-saving leaf disk assay using highly susceptible yellow-fleshed cultivars. The assay enabled 29 molecules/compounds to be screened in relation to their capacity to condition the development of necrosis on leaf disks by treating them before the inoculation. A necrosis index was thereby created. Fosetyl-aluminum, DL-β-Amino-n-butyric acid (BABA) and saccharin (BIT) reduced symptoms significantly, and Fosetyl-aluminum was the most effective. Maxim®, triclopyr and Methyl jasmonate enhanced the necrosis index significantly. S-Methyl 1,2,3-benzothiadiazole-7-carbothioate (BTH) also enhanced symptom severity, though there was no statistical support confirming this. Salicylic acid did not condition symptom development. In vitro investigations into the direct effect of the compounds on Psa highlighted that Fosetyl-aluminum and the polyamines, spermidine and cadaverine, inhibited growth and also had a biocidal effect in a dose-dependent fashion. Salicylic acid and spermine also strongly reduced bacterial growth. Long-term trials with potted plants in the greenhouse confirmed the protective effect of Fosetyl-aluminum and clearly showed that BTH enhances the susceptibility of kiwifruit to Psa. Fosetyl-aluminum significantly decreased symptom expression in kiwifruit plantations (cv. Hayward) subjected to natural Psa infection. Monitoring Psa growth dynamics in the leaf disks by time-point-based Real-Time PCR, showed that treatment with Fosetyl-aluminum restricts bacterial multiplication within the host plant.

丁香假单胞菌PV。猕猴桃（Psa）对全世界的奇异果种植园造成了严重破坏。寻找能够控制这种疾病的化合物仍然至关重要。在这项工作中，我们开发了使用高度易感的黄肉栽培品种的叶盘测定方法，节省了时间和空间。通过在接种前对29种分子/化合物进行处理，可以对29种分子/化合物进行处理，从而调节其在叶盘上坏死的能力，从而对其进行筛选。由此创建了坏死指数。福塞基铝，DL-β-氨基正丁酸（BABA）和糖精（BIT）显着减轻症状，福塞基铝最有效。Maxim®，松果菊和茉莉酸甲酯可显着增强坏死指数。小号-1,2,3-苯并噻二唑-7-硫代甲烷酸甲酯（BTH）也可增强症状的严重程度，尽管尚无统计学支持。水杨酸不能调节症状的发展。对该化合物对Psa的直接作用的体外研究表明，Fetoseyl-Aluminum和多胺，亚精胺和尸胺可以抑制生长，并且还具有剂量依赖性的杀生物作用。水杨酸和精胺也强烈减少细菌的生长。在温室中对盆栽植物进行的长期试验证实了三苯乙胺铝具有保护作用，并清楚地表明BTH增强了奇异果对Psa的敏感性。在遭受天然Psa感染的奇异果种植园中（Cov。Hayward），福塞替基铝显着降低了症状表达。