One-pot synthesis of hyaluronic acid–coated gold nanoparticles as SERS substrate for the determination of hyaluronidase activity,Microchimica Acta

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One-pot synthesis of hyaluronic acid–coated gold nanoparticles as SERS substrate for the determination of hyaluronidase activity
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-10-10 , DOI: 10.1007/s00604-020-04566-3
Wei Wang ₁ , Dan Li ₁ , Yue Zhang ₁ , Wei Zhang ₁ , Pinyi Ma ₁ , Xinghua Wang ₁ , Daqian Song ₁ , Ying Sun ₁

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A novel ultrasensitive surface-enhanced Raman spectroscopy (SERS)-based method was developed for the determination of hyaluronidase (HAase), which was based on hyaluronic acid–coated gold nanoparticles (HA-AuNPs) as a substrate, via a facile one-pot method. The detection mechanism is based on HAase which can hydrolyze HA on HA-AuNPs into hyaluronic acid oligomers, causing the originally uniformly dispersed HA-AuNPs to be disintegrated into many smaller HA-AuNPs. These oligomers in turn increase the surface shielding of AuNPs, resulting in high aggregation tendencies. As a result, the original SERS substrate was disassembled, leading to a weakening of the SERS signal at 1173 cm−1. Malachite green was also used as a Raman probe to detect the change of SERS peak intensity and to quantify HAase. Compared with other methods for the determination of HAase, this method is more convenient and efficient; its determination limit was 0.4 mU mL−1. The recoveries of HAase spiked into human urine samples ranged from 97.2 to 103.9%.

中文翻译：

一锅法合成透明质酸包覆的金纳米粒子作为 SERS 底物用于测定透明质酸酶活性

开发了一种基于超灵敏表面增强拉曼光谱 (SERS) 的新型方法，用于测定透明质酸酶 (HAase)，该方法基于透明质酸包覆的金纳米粒子 (HA-AuNPs) 作为基质，通过简便的一锅法方法。检测机制基于 HAase，可将 HA-AuNPs 上的 HA 水解成透明质酸低聚物，使原本均匀分散的 HA-AuNPs 分解成许多更小的 HA-AuNPs。这些低聚物反过来增加了 AuNPs 的表面屏蔽，导致高聚集趋势。结果，原始的 SERS 基底被分解，导致 1173 cm-1 处的 SERS 信号减弱。孔雀石绿也被用作拉曼探针来检测 SERS 峰强度的变化和量化 HAase。与其他测定HAase的方法相比，该方法更加方便快捷；其测定限为 0.4 mU mL-1。添加到人尿样品中的 HAase 的回收率为 97.2% 到 103.9%。

更新日期：2020-10-10

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