An economical source of nitrogen is one of the major limiting factors for sustainable cadaverine production. The utilization potential of soybean residue for enhanced cadaverine production by engineered Escherichia coli DFC1001 was investigated in this study. The SRH from soybean residue could get the protein extraction rate (PE) of 67.51% and the degree of protein hydrolysis (DH) of 22.49%. The protein molecular weights in SRH were mainly distributed in 565 Da (72.28%) and 1252 Da (17.11%). These proteins with small molecular weights and concentrated molecular weight distribution were favorable to be transformed by engineered E. coli DFC1001, and then SRH replaced completely yeast powder as an only nitrogen source for cadaverine production. The maximum cadaverine productivity was 0.52 g/L/h, achieved with a constant speed feeding strategy in the optimized SRH fermentation medium containing an initial total sugar concentration of 30 g/L and exogenous added minerals, which indicated that soybean residue could be a potential feedstock for economic cadaverine production.

经济的氮源是尸体可持续生产的主要限制因素之一。在这项研究中，研究了大豆残渣对工程化大肠杆菌DFC1001增强尸体生产的利用潜力。大豆渣中的SRH可得到67.51％的蛋白质提取率（PE）和22.49％的蛋白质水解度（DH）。SRH中的蛋白质分子量主要分布在565 Da（72.28％）和1252 Da（17.11％）中。这些分子量小且分子量分布集中的蛋白质有利于工程化大肠杆菌的转化DFC1001，然后SRH完全取代了酵母粉，成为尸胺生产的唯一氮源。在包含初始总糖浓度为30 g / L和外源添加矿物质的优化SRH发酵培养基中，采用恒速进料策略实现的最大尸胺生产率为0.52 g / L / h，这表明大豆残渣可能是潜在的用于尸体生产的原料。