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Understanding the molecular basis on the biological suppression of bacterial leaf blight of anthurium exerted by Bacillus subtilis (BIO3) through proteomic approach
3 Biotech ( IF 2.8 ) Pub Date : 2020-10-10 , DOI: 10.1007/s13205-020-02456-2
S Nakkeeran 1 , M Suganyadevi 1 , S Rajamanickam 1
Affiliation  

We attempted to study the antibacterial activity of rhizospheric Bacillus spp., to curb the bacterial blight of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad). Twenty-eight bacterial isolates from rhizospheric regions were identified as different Bacillus spp. and Ochrobactrum sp. using 16S rRNA gene sequencing. B. subtilis BIO3 effectively inhibited the growth of Xad up to 1450.7 mm2, and extracted volatile organic metabolites from the isolate BIO3 inhibited the growth of Xad up to 1024 mm2. Tritrophic interaction of anthurium leaves bacterized with B. subtilis BIO3 and challenged with Xad resulted in the expression of 12 unique proteins compared to untreated control. Mascot Peptide Mass Fingerprint-based identification indicated that one was glutathione peroxidase, involved in defence mechanism, other six proteins were identified as leghemoglobin II, CTP synthase-like, predicted protein (Physcomitrella patens), centromere-associated protein E, grain size protein, and five proteins were hypothetical proteins. Foliar application with 1% liquid formulations (108 CFU/ml) of B. subtilis BIO3 significantly suppressed the bacterial leaf blight of anthurium up to 78% over untreated control and also increased the stem length and flower yield.



中文翻译:

通过蛋白质组学方法了解枯草芽孢杆菌(BIO3)对红掌细菌性叶枯病生物抑制的分子基础

我们试图研究根际芽孢杆菌的抗菌活性,以遏制由Xanthomonas axonopodis pv.引起的红掌细菌性枯萎病。dieffenbachiaeXAD)。来自根际区域的 28 种细菌分离物被鉴定为不同的芽孢杆菌属。和Ochrobactrum sp。使用 16S rRNA 基因测序。B. subtilis BIO3 可有效抑制Xad 的生长达 1450.7 mm 2,从分离株 BIO3 中提取的挥发性有机代谢物可抑制Xad 的生长达 1024 mm 2 。. 与未处理的对照相比,用枯草芽孢杆菌BIO3 杀菌并用Xad攻击的红掌叶的三营养相互作用导致了 12 种独特蛋白质的表达。Mascot Peptide Mass Fingerprint 鉴定表明,一种是谷胱甘肽过氧化物酶,参与防御机制,其他六种蛋白质被鉴定为豆血红蛋白II、CTP 合成酶样、预测蛋白(Physcomitrella patens)、着丝粒相关蛋白E、颗粒大小蛋白、五种蛋白质是假设蛋白质。叶面喷施 1%枯草芽孢杆菌液体制剂 (10 8  CFU/ml) 与未处理的对照相比,BIO3 显着抑制红掌细菌性叶枯病高达 78%,并且还增加了茎长和花产量。

更新日期:2020-10-11
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