Skip to main content
SpringerLink
Log in

Understanding the molecular basis on the biological suppression of bacterial leaf blight of anthurium exerted by Bacillus subtilis (BIO3) through proteomic approach

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

We attempted to study the antibacterial activity of rhizospheric Bacillus spp., to curb the bacterial blight of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad). Twenty-eight bacterial isolates from rhizospheric regions were identified as different Bacillus spp. and Ochrobactrum sp. using 16S rRNA gene sequencing. B. subtilis BIO3 effectively inhibited the growth of Xad up to 1450.7 mm2, and extracted volatile organic metabolites from the isolate BIO3 inhibited the growth of Xad up to 1024 mm2. Tritrophic interaction of anthurium leaves bacterized with B. subtilis BIO3 and challenged with Xad resulted in the expression of 12 unique proteins compared to untreated control. Mascot Peptide Mass Fingerprint-based identification indicated that one was glutathione peroxidase, involved in defence mechanism, other six proteins were identified as leghemoglobin II, CTP synthase-like, predicted protein (Physcomitrella patens), centromere-associated protein E, grain size protein, and five proteins were hypothetical proteins. Foliar application with 1% liquid formulations (108 CFU/ml) of B. subtilis BIO3 significantly suppressed the bacterial leaf blight of anthurium up to 78% over untreated control and also increased the stem length and flower yield.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Athukorala SNP, Fernando WGD, Rashid KY (2009) Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry. Can J Microbiol 55:1021–1032

    Article  CAS  Google Scholar 

  • Aysan Y, Sahin F (2003) First report of bacterial blight of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae in Turkey. New Dis Rep 7:5

    Google Scholar 

  • Balan S, Sajeesh PK, Abraham K (2014) Morphological and biochemical characterization of Xanthomonas axonopodis pv. dieffenbachiae (McCulloch & Pirone) causing bacterial blight of anthurium (Schott.). Trends in Biosciences 7(8):636–640

    Google Scholar 

  • Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized Single disk method. Am J Clin Pathol 45:493–496

    Article  CAS  Google Scholar 

  • Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93–99

    Article  CAS  Google Scholar 

  • Cano RJ, Borucki MK, Highby-Schweitzer M, Poinar HN, Poinar GO, Pollard KJ (1994) Bacillus DNA in fossil bees: an ancient symbiosis. Appl Environ Microbiol 60(6):2164–2167

    Article  CAS  Google Scholar 

  • Cheng J, Jaiswal KS, Yang SH, Suh JW (2016) Endophytic Bacillus subtilis MJMP2 from Kimchi inhibits Xanthomonas oryzae pv. oryzae, the pathogen of rice bacterial blight disease. J Appl Biol Chem 59(2):149–154

    Article  CAS  Google Scholar 

  • Das R, Mondal B, Mondal P, Khatua DC, Mukherjee N (2014) Biological management of citrus canker on acid lime through Bacillus subtilis (S-12) in West Bengal, India. J Biopest 7:38–41

    Google Scholar 

  • Dhanya S, Sreekumar J, Jisha MS (2016) Evaluation of antagonistic potential and induction of flowering in Anthurium andreanum L. by indigenous Pseudomonas taiwanensis. Int J Agric Innov Res 4(4):760–766

    Google Scholar 

  • Dheepa R, Vinodkumar S, Renukadevi P, Nakkeeran S (2016) Phenotypic and molecular characterization of chrysanthemum white rust pathogen Puccinia horiana (Henn) and the effect of liquid based formulation of Bacillus spp. for the management of chrysanthemum white rust under protected cultivation. Biol Control. https://doi.org/10.1016/j.biocontrol.2016.09.006

    Article  Google Scholar 

  • Gomez KA, Gomez AA (1984) Statistical procedure for agricultural research. Wiley, New York

    Google Scholar 

  • Isabel M, Jordi C, Emilio M (2011) Antimicrobial peptide genes in Bacillus strains from plant environments. Int Microbiol 14:213–223

    Google Scholar 

  • Knapp JE, Chandlee JM (1996) RNA/DNA mini-prep from a single sample of orchid tissue. Biotechniques 21:54–56

    Article  CAS  Google Scholar 

  • Kumsingkaew S, Akarapisan A (2014) Efficiency of Bacillus subtilis EPB14 as Biocontrol to control bacterial leaf blight of anthurium. Int J Agric Technol 10(3):755–766

    Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947–2948

    Article  CAS  Google Scholar 

  • Li SB, Fang M, Zhou RC, Huang J (2012) Characterization and evaluation of the endophyte Bacillus B014 as a potential biocontrol agent for the control of Xanthomonas axonopodis pv. dieffenbachiae-Induced blight of Anthurium. Biol Control 63(1):9–16

    Article  CAS  Google Scholar 

  • Li SB, Xu SR, Zhang RN, Liu Y, Zhou RC (2015) The antibiosis action and rice induced resistance, mediated by a lipopeptide from Bacillus amyloliquefaciens B014, in controlling rice disease caused by Xanthomonas oryzae pv. oryzae. J Microbiol Biotechnol 26(4):748–756. https://doi.org/10.4014/jmb.1510.10072

    Article  CAS  Google Scholar 

  • Lian L, Xie L, Zheng L, Lin Q (2011) Induction of systemic resistance in tobacco against Tobacco mosaic virus by Bacillus spp. Biocontrol Sci Technol 21:281–292

    Article  Google Scholar 

  • Mohan Kumar SP, Chowdappa P, Krishna V (2015) Development of seed coating formulation using consortium of Bacillus subtilis OTPB1 and Trichoderma harzianum OTPB3 for plant growth promotion and induction of systemic resistance in field and horticultural crops. Indian Phytopath 68(1):25–31

    Google Scholar 

  • Nadia MH, Hajri A, Boureau T, Darrasse A, Durand K, Brin C, Fischer-LeSaux M, Manceau C, Poussier S, Pruvost O, Lemaire C, Marie-Agnes J (2013) Evolutionary history of the plant pathogenic bacterium Xanthomonas axonopodis. PLoS ONE 8(3):e58474

    Article  Google Scholar 

  • Nakkeeran S, Surya T, Vinodkumar S (2019) Antifungal potential of plant growth promoting Bacillus species against blossom blight of rose. J Plant Growth Regul. https://doi.org/10.1007/s00344-019-09966-1

    Article  Google Scholar 

  • Nishijima WT (1988) Anthurium blight: An overview. In: A. M. Alvarez (Eds) Proceedings of the first anthurium blight conference, Honolulu, USA, pp. 6–8

  • Prabhukarthikeyan SR, Manikandan R, Durgadevi D, Keerthana U, Harish S, Karthikeyan G, Raguchander T (2017) Bio-suppression of turmeric rhizome rot disease and understanding the molecular basis of tripartite interaction among Curcuma longa, Pythium aphanidermatum and Pseudomonas fluorescens. Biol Control 111:23–31

    Article  CAS  Google Scholar 

  • Prakasha DP, Ramya G, Srinivasalu GB (2017) Morphogenetic responses in Anthurium andreanum (Hort) cultivars. Curr Agric Res J 5(1):135–140

    Article  Google Scholar 

  • Prapagdee B, Tharasaithong L, Nanthaphot R, Paisitwiroj C (2012) Efficacy of crude extract of antifungal compounds produced from Bacillus subtilis on prevention of anthracnose disease in Dendrobium Orchid. Environ Asia 5(1):32–38

    Google Scholar 

  • Rajamanickam S, Karthikeyan G, Kavino M, Manoranjitham SK (2018) Biohardening of micro-propagated banana using endophytic bacteria induces plant growth promotion and restrain the rhizome rot disease caused by Pectobacterium carotovorum subsp. carotovorum. Sci Hortic 231:179–187

    Article  Google Scholar 

  • Sathyanarayana N, Reddy OR, Latha S, Rajak RL (1998) Interception of Xanthomonas campestris pv Dieffenbachiae on Anthurium plants from the Netherlands. Plant Dis 82(2):262

    Article  CAS  Google Scholar 

  • Saveetha K, Karthiba L, Raveendran M, Saravanakumar D, Suresh S, Raguchander T, Balasubramanian P, Samiyappan R (2009) Understanding the molecular basis of plant growth promotional effect of Pseudomonas fluorescens on rice through protein profiling. Proteome Sci 7:1–8

    Article  Google Scholar 

  • Shen S, Jing Y, Kuang T (2003) Proteomics approach to identify wound response related proteins from rice leaf sheath. Proteomics 3:527–535

    Article  CAS  Google Scholar 

  • Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68:850–858

    Article  CAS  Google Scholar 

  • Shrestha BK, Karki HS, Groth DE, Nootjarin JN, Ham JH (2016) Biological control activities of rice-associated Bacillus sp. strains against sheath blight and bacterial panicle blight of rice. PLoS ONE 1(1):e0146764

    Article  Google Scholar 

  • Srinivasan K, Mathivanan N (2011) Plant growth promoting microbial consortia mediated classical biocontrol of sunflower necrosis virus disease. J Biopest 4(1):65–72

    Google Scholar 

  • Suganyadevi M, Nakkeeran S, Renukadevi P (2015) Molecular characterization of plant quarantine pathogen Xanthomonas axonopodis pv dieffenbachiae causing bacterial blight in anthurium and its management. J Mycol Plant Pathol 45(4):336–341

    Google Scholar 

  • Teixeira da Silva JA, Dobranszki J, Winarto B, Zeng S (2015) Anthurium in vitro: a review. Sci Hortic 186:266–298

    Article  Google Scholar 

  • Vinodkumar S, Nakkeeran S, Renukadevi P, Malathi VG (2017) Biocontrol potentials of antimicrobial peptide producing Bacillus species: multifaceted antagonists for the management of stem rot of carnation caused by Sclerotinia sclerotiorum. Front Microbiol 8:1–13

    Article  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173(2):697–703

    Article  CAS  Google Scholar 

  • Suganyadevi M, Renukadevi P, Nakkeeran S (2016) Efficacy of biocontrol agents and bactericides for the management of bacterial blight incited by Xanthomonas axonopodis pv. dieffenbachiae in Anthurium andraeanum. Int J Plant Protect 9(1):292–296

    Article  Google Scholar 

Download references

Acknowledgement

The author acknowledges DST-FIST, Department of Science and Technology, Government of India, New Delhi, for the infrastructure facilities for conducting the experiments.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    S. Nakkeeran, M. Suganyadevi & S. Rajamanickam

Authors
  1. S. Nakkeeran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Suganyadevi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Rajamanickam
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

NS and RS have performed the laboratory experiments, glasshouse experiment, statistical analysis, data recording and writing the original draft. SM has performed the proteomic study. All authors proofread and reviewed the manuscript.

Corresponding author

Correspondence to S. Nakkeeran.

Ethics declarations

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 236 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nakkeeran, S., Suganyadevi, M. & Rajamanickam, S. Understanding the molecular basis on the biological suppression of bacterial leaf blight of anthurium exerted by Bacillus subtilis (BIO3) through proteomic approach. 3 Biotech 10, 468 (2020). https://doi.org/10.1007/s13205-020-02456-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-020-02456-2

Keywords

Search

Navigation