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Contents of Myelin Basic Protein and Autoantibodies against Brain Proteins in the Experimental Antiphospholipid Syndrome
Neurophysiology ( IF 0.6 ) Pub Date : 2020-03-01 , DOI: 10.1007/s11062-020-09860-7
O. Z. Yaremchuk

We examined the content of autoantibodies against brain proteins, content of myelin basic protein (MBP), and level of NO synthesis in the cerebellum and cerebral hemispheres on day 18 of pregnancy in BALB/c mice with the experimental antiphospholipid syndrome (APS); the effects of L-arginine on the above indices were also evaluated. As was found, the contents of autoantibodies against brain proteins having the molecular masses 120, 150, and > 170 kDa were greater than in the control. Under APS conditions, the amount of eNOS-produced NO was relatively insufficient; this was observed against the background of total hyperproduction of NO synthesized by iNOS in blood serum. In APS mice, the contents of stable NO metabolites, NO2– and NO3–, in the cerebellum were higher, while these levels in the cerebral hemispheres were lower with respect to the control. There were reasons to believe that the effects of L-arginine under APS conditions of and cerebral dysfunction are provided not only at the expense of influences upon the NO system, but also via antioxidant and cytoprotective properties of this amino acid. In pregnant APS mice, the content of MBP (95–110 kDa) in the cerebellum and that of MBP (18.4 kDa) in the cerebral hemispheres were greater than in the control. In APS animals, administration of L-arginine provided increase in the content of MBP (18.4 kDa) in the cerebral hemispheres compared with the respective index with no treatment. Our results show that the remyelination processes in animals with the APS are activated; this may be interpreted as a compensatory response to the injury.

中文翻译:

实验性抗磷脂综合征髓鞘碱性蛋白和脑蛋白自身抗体的含量

我们检测了具有实验性抗磷脂综合征 (APS) 的 BALB/c 小鼠在怀孕第 18 天的脑蛋白自身抗体的含量、髓鞘碱性蛋白 (MBP) 的含量以及小脑和大脑半球的 NO 合成水平;还评估了 L-精氨酸对上述指标的影响。正如所发现的,针对分子质量为 120、150 和 > 170 kDa 的脑蛋白的自身抗体的含量高于对照。在APS条件下,eNOS产生的NO量相对不足;这是在血清中由 iNOS 合成的 NO 总量过度产生的背景下观察到的。在 APS 小鼠中,小脑中稳定的 NO 代谢物 NO2- 和 NO3- 的含量较高,而大脑半球中的这些水平与对照相比较低。有理由相信 L-精氨酸在 APS 和脑功能障碍条件下的作用不仅以对 NO 系统的影响为代价,而且通过该氨基酸的抗氧化和细胞保护特性提供。在怀孕的 APS 小鼠中,小脑中 MBP (95-110 kDa) 和大脑半球 MBP (18.4 kDa) 的含量高于对照组。在 APS 动物中,与未处理的相应指数相比,施用 L-精氨酸增加了大脑半球 MBP (18.4 kDa) 的含量。我们的结果表明,APS 动物的髓鞘再生过程被激活;这可以解释为对伤害的补偿性反应。有理由相信 L-精氨酸在 APS 和脑功能障碍条件下的作用不仅以对 NO 系统的影响为代价,而且通过该氨基酸的抗氧化和细胞保护特性提供。在怀孕的 APS 小鼠中,小脑中 MBP (95-110 kDa) 和大脑半球 MBP (18.4 kDa) 的含量高于对照组。在 APS 动物中,与未处理的相应指数相比,L-精氨酸的施用增加了大脑半球中 MBP (18.4 kDa) 的含量。我们的结果表明,APS 动物的髓鞘再生过程被激活;这可以解释为对伤害的补偿性反应。有理由相信 L-精氨酸在 APS 和脑功能障碍条件下的作用不仅以对 NO 系统的影响为代价,而且通过该氨基酸的抗氧化和细胞保护特性提供。在怀孕的 APS 小鼠中,小脑中 MBP (95-110 kDa) 和大脑半球 MBP (18.4 kDa) 的含量高于对照组。在 APS 动物中,与未处理的相应指数相比,施用 L-精氨酸增加了大脑半球 MBP (18.4 kDa) 的含量。我们的结果表明,APS 动物的髓鞘再生过程被激活;这可以解释为对伤害的补偿性反应。
更新日期:2020-03-01
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