Actinidia deliciosa is a commercially important plant receiving recognition because of its high nutritive value. This study presents an efficient protocol for the hardening of in vitro raised Actinidia deliciosa plants using a hydroponic method. Leaf explants inoculated on Murashige and Skoog (MS) medium supplemented with 5.00 μM 6-benzylaminopurine (BAP) and 1.00 μM α-naphthaleneacetic acid (NAA) resulted in 5.28 ± 0.14 shoots per explants and 8.33 ± 1.80 cm average shoot length. Rooting was achieved through half-strength MS medium supplemented with 1.5 μM indolebutyric acid (IBA) and 0.6 μM BAP. Maximum root number (12.76 ± 1.08) and 6.78 ± 0.25 cm average root length were recorded from plantlets using half-strength MS medium supplemented with 1.5 μM indolebutyric acid (IBA) and 0.6 μM BAP. In hydroponic system, an average root length of 22.40 ± 0.59 cm, average root number of 21.50 ± 1.24, average leaf number of 4.50 ± 0.40, and average shoot length of 9.71 ± 0.29 cm were observed in Hoagland & Arnon solution. The early development of shoots, roots, and leaves through hydroponics was advantageous in establishment of micropropagated plants in a greenhouse. Complete 100% plant survival was found by following proper acclimatization using hydroponic method. The study underlines the efficient hardening of micropropagated plants of A. deliciosa through hydroponic technique in Himalayan region.

猕猴桃因其高营养价值而成为获得认可的商业上重要的植物。这项研究提出了一种有效的方案，以加强体外培养的猕猴桃植物使用水培法。在补充了5.00μM6-苄氨基嘌呤（BAP）和1.00μMα-萘乙酸（NAA）的Murashige和Skoog（MS）培养基上接种的叶子外植体，每个外植体的芽长为5.28±0.14，平均芽长为8.33±1.80 cm。通过添加1.5μM吲哚丁酸（IBA）和0.6μMBAP的半强度MS培养基实现生根。使用补充了1.5μM吲哚丁酸（IBA）和0.6μMBAP的半强度MS培养基从小植株上记录了最大根数（12.76±1.08）和6.78±0.25 cm平均根长。在水耕系统中，在Hoagland＆Arnon溶液中观察到平均根长为22.40±0.59 cm，平均根数为21.50±1.24，平均叶数为4.50±0.40，平均枝长为9.71±0.29 cm。芽的早期发育 根和叶通过水培法有利于在温室中建立微繁殖植。通过使用水耕法进行适当的驯化，可以发现100％的植物完全存活。该研究强调了微繁殖植物的有效硬化通过水培技术在喜马拉雅地区的A. deliciosa。