Investigation of Zat12-induced biochemical responses was carried out in Zat12 transgenic lines (Z-8-12 1A, Z-8-12 1B, Z-8-19 and Z-15-10) generated from wheat cv. PBW621 in response to pathogen infection and salinity stress (150 and 250 mM NaCl). Pathogen infection (after 48 h) led to a significant increase in ascorbate levels and activity of ascorbate peroxidase (APX) along with decreased hydrogen peroxide (H2O2) and malondialdehyde content in Zat12 transgenics as compared to PBW621. However, under salinity stress (250 mM NaCl), a marked elevation in H2O2 and malondialdehyde content followed by low content of ascorbate and ascorbate peroxidase activity was found in PBW621 as compared to transgenics. The results thus emphasized that the transformed lines combat abiotic and biotic stress by accumulating higher ascorbate via increased activity of APX triggered by Zat12 gene, suggesting low membrane injury and higher antioxidant potential in these lines. Histochemical localization pattern also revealed correlation of H2O2 with activity of ascorbate peroxidase in Zat12 transgenics.

中文翻译：

---

Zat12小麦转基因作物病原侵染-盐分胁迫的生化反应

在从小麦品种产生的 Zat12 转基因品系（Z-8-12 1A、Z-8-12 1B、Z-8-19 和 Z-15-10）中进行了 Zat12 诱导的生化反应的研究。PBW621 响应病原体感染和盐度胁迫（150 和 250 mM NaCl）。与 PBW621 相比，病原体感染（48 小时后）导致 Zat12 转基因中抗坏血酸水平和抗坏血酸过氧化物酶 (APX) 活性显着增加，同时降低过氧化氢 (H2O2) 和丙二醛含量。然而，在盐度胁迫（250 mM NaCl）下，与转基因相比，在 PBW621 中发现 H2O2 和丙二醛含量显着升高，随后抗坏血酸和抗坏血酸过氧化物酶活性含量低。因此，结果强调转化的品系通过由 Zat12 基因触发的 APX 活性增加而积累更高的抗坏血酸来对抗非生物和生物胁迫，表明这些品系的膜损伤低，抗氧化潜力更高。组织化学定位模式还揭示了 H2O2 与 Zat12 转基因中抗坏血酸过氧化物酶活性的相关性。