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Biochemical responses of pathogen infestation-cum-salinity stress in Zat12 wheat transgenics

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Abstract

Investigation of Zat12-induced biochemical responses was carried out in Zat12 transgenic lines (Z-8-12 1A, Z-8-12 1B, Z-8-19 and Z-15-10) generated from wheat cv. PBW621 in response to pathogen infection and salinity stress (150 and 250 mM NaCl). Pathogen infection (after 48 h) led to a significant increase in ascorbate levels and activity of ascorbate peroxidase (APX) along with decreased hydrogen peroxide (H2O2) and malondialdehyde content in Zat12 transgenics as compared to PBW621. However, under salinity stress (250 mM NaCl), a marked elevation in H2O2 and malondialdehyde content followed by low content of ascorbate and ascorbate peroxidase activity was found in PBW621 as compared to transgenics. The results thus emphasized that the transformed lines combat abiotic and biotic stress by accumulating higher ascorbate via increased activity of APX triggered by Zat12 gene, suggesting low membrane injury and higher antioxidant potential in these lines. Histochemical localization pattern also revealed correlation of H2O2 with activity of ascorbate peroxidase in Zat12 transgenics.

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Abbreviations

APX:

Ascorbate peroxidase

DAB:

3,3-Diaminobenzidine

MDA:

Malondialdehyde

ROS:

Reactive oxygen species

References

  • Afzal F, Chaudhari SK, Gul A, Farooq A, Ali H, Nisar S, Mujeeb-Kazi A (2015) Bread wheat (Triticum aestivum L.) under biotic and abiotic stresses: an overview. In: Crop production and global environmental issues. Springer, Cham, pp 293–317

  • Anjum NA, Duarte AC, Pereira E, Ahmad I (2015) Juncus maritimus root biochemical assessment for its mercury stabilization potential in Ria de Aveiro coastal lagoon (Portugal). Environ Sci Pollut Res 22:2231–2238

    Article  CAS  Google Scholar 

  • Davletova S, Rizhsky L, Liang H, Shengqiang Z, Oliver DJ, Coutu J, Shulaev V, Schlauch K, Mittler R (2005a) Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17:268–281

    Article  CAS  Google Scholar 

  • Davletova S, Schlauch K, Coutu J, Mittler R (2005b) The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiol 139:847–856

    Article  CAS  Google Scholar 

  • Duveiller E, Maraite H (1993) Study of yield loss due to Xanthomonas campestris pv. undulosa in wheat under high rainfall temperate conditions. J Plant Dis Protect 100:453–459

    Google Scholar 

  • Forster RL, Schaad NW (1988) Control of black chaff of wheat with seed treatment and a foundation seed health program. Plant Dis 72:935–938

    Article  Google Scholar 

  • Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue culture media. In vitro 12:473–478

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  Google Scholar 

  • Johnson SM, Doherty SJ, Croy RRD (2003) Biphasic superoxide generation in potato tubers: a self amplifying response to stress. Plant Physiol 13:1440–1449

    Article  Google Scholar 

  • Kaur R (2014) Genetic transformation of bread wheat (Triticum aestivum L.) by ‘particle gun and Agrobacterium-mediated approaches. PhD dissertation, Punjab Agricultural University, Ludhiana, India

  • Kazempour MN, Kheyrgoo M, Pedramfar H, Rahimian H (2010) Isolation and identification of bacterial glum blotch and leaf blight on wheat (Triticum aestivum L.) in Iran. Afr J Biotech 9:2860–2865

    CAS  Google Scholar 

  • Kissoudis C, van de Wiel C, Visser RGF, van der Linden G (2014) Enhancing crop resilience to combined abiotic and biotic stress through the dissection of physiological and molecular crosstalk. Front Plant Sci 5:207

    Article  Google Scholar 

  • Kumar S, Beena AS, Awana M, Singh A (2017) Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Front Plant Sci 8:1151

    Article  Google Scholar 

  • Law M, Charles S, Halliwell B (1983) Glutathione and ascorbic acid on spinach (Spincaciaoleracea) chloroplasts: the effect of hydrogen peroxide and paraquat. J Biochem 210:889–903

    Article  Google Scholar 

  • Low LY, Yang SK, Kok DX, Ong-Abdullah J, Tan NP, Lai KS (2018) Transgenic plants: gene constructs, vector and transformation method. In: New visions in plant science. https://doi.org/10.5772/intechopen.79369

  • Munns R, Gilliham M (2015) Salinity tolerance of crops—what is the cost? New Phytol 208:668–673

    Article  CAS  Google Scholar 

  • Nankano Y, Asada K (1974) Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    Google Scholar 

  • Patterson BD (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492

    Article  CAS  Google Scholar 

  • Rizhsky L, Davletova S, Liang H, Mittler R (2004) The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J BiolChem 279:11736–11743

    CAS  Google Scholar 

  • Roy S, Arora A, Chinnusamy V, Singh VP (2017) Endogenous reduced ascorbate: an indicator of plant water deficit stress in wheat. Indian J Plant Physiol 22:365–368

    Article  CAS  Google Scholar 

  • Saghai-Maroof MA, Biyashev RM, Yang GR, Zhang Q, Allard RW (1984) Extraordinarily polymorphic microsatellite DNA in barley species diversity, chromosomal locations and population dynamics. Proc Natl Acad Sci USA 91:546–547

    Google Scholar 

  • Slesak I, Libik M, Karpinska B, Karpinski S, Miszalski Z (2007) The role of hydrogen peroxide in regulation of plant metabolism and cellular signaling in response to environmental stresses. Acta Biochim Polon 54:39–50

    Article  CAS  Google Scholar 

  • Tan BL, Norhaizan ME, Liew WPP, SulaimanRahman H (2018) Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Front Pharmacol 9:1162

    Article  Google Scholar 

  • Thaler JS, Bostock RM (2004) Interactions between abscisic-acidmediated responses and plant resistance to pathogens and insects. Ecology 85:48–58

    Article  Google Scholar 

  • Thordal-Christensen H, Zang ZW, Yand CDB (1997) Subcellular localization of H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11:1187–1194

    Article  CAS  Google Scholar 

  • Toben H, Mavridis A, Rudolph KWE (1991) On the occurrence of basal glume root wheat and barley caused by Pseudomonas syringaepv. atrofaciens in West Germany. J Plant Dis Protect 98:225–235

    Google Scholar 

  • Von Kietzell J, Rudolph K (1997) “Wheat diseases caused by Pseudomonas syringae pathovars,” in the bacterial diseases of wheat. In: Concepts and methods of disease management, No. 633.11932 DUV, pp 49–57

  • Wilkins T, Smart L (1996) Isolation of RNA from plant tissue. In: A laboratory guide to RNA: isolation, analysis, and synthesis, vol 43, pp 21–42

  • World Agricultural Production (2017) United States Department of Agriculture. Foreign agricultural service circular series, WAP 12–17.

  • Xie J, Dai Y, Mu H, De Y, Chen H, Wu Z, Ren W (2016) Physiological and biochemical responses to NACl salinity stress in three Roegneria (Poaceae) species. Pakistan J Bot 48:2215–2222

    CAS  Google Scholar 

  • Xie M, Sun J, Gong D, Kong Y (2019) The Roles of Arabidopsis C1–2i Subclass of C2H2-type zinc-finger transcription factors. Genes 10:653

    Article  CAS  Google Scholar 

  • Xu J, Zhou Y, Xu Z, Chen Z, Duan L (2020) Combining physiological and metabolic analysis to unravel the regulations of coronatine alleviating water stress in tobacco (Nicotianatobacum L.). Biomol 10(1):99

    Article  CAS  Google Scholar 

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Acknowledgement

We thank Dr. Navtej Singh Bains generously for his guidance throughout the work.

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Authors and Affiliations

  1. Department of Biochemistry, Punjab Agricultural University, Ludhiana, Punjab, India

    Manpreet Kaur, Bavita Asthir & Gurpreet Kaur

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  1. Manpreet Kaur
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  2. Bavita Asthir
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  3. Gurpreet Kaur
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Correspondence to Bavita Asthir.

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Kaur, M., Asthir, B. & Kaur, G. Biochemical responses of pathogen infestation-cum-salinity stress in Zat12 wheat transgenics. CEREAL RESEARCH COMMUNICATIONS 49, 55–63 (2021). https://doi.org/10.1007/s42976-020-00091-4

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  • DOI: https://doi.org/10.1007/s42976-020-00091-4

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