One of the reasons hindering large-scale application of sophorolipids (SLs) is high production cost. In this study, six recombinant strains of Starmerella bombicola, sbEG1, sbEG2, sbCBH1, sbCBH1–2, sbBGL1, and sbCBH2 expressing cellulase genes eg1, eg2, cbh, cbh1–2, bgl1, and cbh2 from Penicillium oxalicum were respectively constructed. Four strains showed cellulase activities and were co-cultivated in fermentation media containing 2% glucose, 1% Regenerated Amorphous Cellulose (RAC), 2% glucose, and 1% RAC, respectively. After 7 days’ cultivation, concentration of SLs in medium with 1% RAC (g/L) reached 1.879 g/L. When 2% glucose and 1% of RAC were both contained, the titer of SLs increased by 39.5% than that of control strain and increased by 68.8% than that in the medium with only 2% glucose. Results demonstrated that cellulase genes from filamentous fungi in S. bombicola can function to degrade lignocellulosic cellulose to produce SLs.

阻碍槐糖脂（SLs）大规模应用的原因之一是高生产成本。在这项研究中，六个重组菌株Starmerella丝酵母菌，SB EG1，sbEG2，SB CBH1，SB CBH1-2，SB BGL1，和SB CBH2表达纤维素酶基因EG1，EG2，CBH，cbh1-2，BGL1和CBH2从草酸青霉分别建造。四个菌株显示纤维素酶活性，并分别在包含2％葡萄糖，1％再生无定形纤维素（RAC），2％葡萄糖和1％RAC的发酵培养基中共培养。培养7天后，含1％RAC（g / L）的培养基中SL的浓度达到1.879 g / L。当同时包含2％的葡萄糖和1％的RAC时，SLs的滴度比对照菌株的滴度提高39.5％，比仅含2％葡萄糖的培养基的滴度提高68.8％。结果表明，来自S. bombicola中丝状真菌的纤维素酶基因可以起到降解木质纤维素纤维素的作用，从而产生SL。