Abstract
One of the reasons hindering large-scale application of sophorolipids (SLs) is high production cost. In this study, six recombinant strains of Starmerella bombicola, sbEG1, sbEG2, sbCBH1, sbCBH1–2, sbBGL1, and sbCBH2 expressing cellulase genes eg1, eg2, cbh, cbh1–2, bgl1, and cbh2 from Penicillium oxalicum were respectively constructed. Four strains showed cellulase activities and were co-cultivated in fermentation media containing 2% glucose, 1% Regenerated Amorphous Cellulose (RAC), 2% glucose, and 1% RAC, respectively. After 7 days’ cultivation, concentration of SLs in medium with 1% RAC (g/L) reached 1.879 g/L. When 2% glucose and 1% of RAC were both contained, the titer of SLs increased by 39.5% than that of control strain and increased by 68.8% than that in the medium with only 2% glucose. Results demonstrated that cellulase genes from filamentous fungi in S. bombicola can function to degrade lignocellulosic cellulose to produce SLs.
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This study was funded by the National Natural Science Foundation of China (No. 31971387) and Major Program of Natural Science Foundation of Shandong Province (No. ZR2019ZD19).
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Yue Li, Na Gao, Xinyu Zhang, and Guoqin Zhao contributed equally to this manuscript.
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Li, Y., Gao, N., Zhang, X. et al. Sophorolipid Production Using Lignocellulosic Biomass by Co-culture of Several Recombinant Strains of Starmerella bombicola with Different Heterologous Cellulase Genes from Penicillum oxalicum. Appl Biochem Biotechnol 193, 377–388 (2021). https://doi.org/10.1007/s12010-020-03433-4
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DOI: https://doi.org/10.1007/s12010-020-03433-4