1-Deoxynojirumycin (1-DNJ) is a representative iminosugar with α-glucosidase inhibition (AGI) activity. In this study, the full genome sequencing of 1-DNJ-producing Bacillus velezensis K26 was performed. The genome consists of a circular chromosome (4,047,350 bps) with two types of putative virulence factors, five antibiotic resistance genes, and seven secondary metabolite biosynthetic gene clusters. Genomic analysis of a wide range of Bacillus species revealed that a 1-DNJ biosynthetic gene cluster was commonly present in four Bacillus species (B. velezensis, B. pseudomycoides, B. amyloliquefaciens, and B. atrophaeus). In vitro experiments revealed that the increased mRNA expression levels of the three 1-DNJ biosynthetic genes were closely related to increased AGI activity. Genomic comparison and alignment of multiple gene sequences indicated the conservation of the 1-DNJ biosynthetic gene cluster in each Bacillus species. This genomic analysis of Bacillus species having a 1-DNJ biosynthetic gene cluster could provide a basis for further research on 1-DNJ-producing bacteria.

1-Deoxynojirumycin (1-DNJ) 是具有 α-葡萄糖苷酶抑制 (AGI) 活性的代表性亚氨基糖。在这项研究中，对产生 1-DNJ 的Bacillus velezensis K26 进行了全基因组测序。基因组由一个环状染色体（4,047,350 bps）组成，具有两种推定的毒力因子、五个抗生素抗性基因和七个次级代谢物生物合成基因簇。对多种芽孢杆菌的基因组分析表明，1-DNJ 生物合成基因簇通常存在于四种芽孢杆菌（B. velezensis、B. pseudomycoides、B. amyloliquefaciens和B. atrophaeus）中。体外实验表明，三种 1-DNJ 生物合成基因的 mRNA 表达水平的增加与 AGI 活性的增加密切相关。多个基因序列的基因组比较和比对表明每个芽孢杆菌属物种中 1-DNJ 生物合成基因簇的保守性。这种对具有 1-DNJ 生物合成基因簇的芽孢杆菌物种的基因组分析可以为进一步研究产生 1-DNJ 的细菌提供基础。