Elsevier

Genomics

Volume 113, Issue 1, Part 2, January 2021, Pages 647-653
Genomics

Original Article
Genome analysis of 1-deoxynojirimycin (1-DNJ)-producing Bacillus velezensis K26 and distribution of Bacillus sp. harboring a 1-DNJ biosynthetic gene cluster

https://doi.org/10.1016/j.ygeno.2020.09.061Get rights and content
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Highlights

  • Genomic information of Bacillus velezensis K26 with α-glucosidase inhibition activity was provided.

  • The 1-deoxynojirimycin (1-DNJ) biosynthetic gene cluster was mainly found in specific Bacillus species.

  • The 1-DNJ biosynthetic cluster was conserved within each Bacillus species.

Abstract

1-Deoxynojirumycin (1-DNJ) is a representative iminosugar with α-glucosidase inhibition (AGI) activity. In this study, the full genome sequencing of 1-DNJ-producing Bacillus velezensis K26 was performed. The genome consists of a circular chromosome (4,047,350 bps) with two types of putative virulence factors, five antibiotic resistance genes, and seven secondary metabolite biosynthetic gene clusters. Genomic analysis of a wide range of Bacillus species revealed that a 1-DNJ biosynthetic gene cluster was commonly present in four Bacillus species (B. velezensis, B. pseudomycoides, B. amyloliquefaciens, and B. atrophaeus). In vitro experiments revealed that the increased mRNA expression levels of the three 1-DNJ biosynthetic genes were closely related to increased AGI activity. Genomic comparison and alignment of multiple gene sequences indicated the conservation of the 1-DNJ biosynthetic gene cluster in each Bacillus species. This genomic analysis of Bacillus species having a 1-DNJ biosynthetic gene cluster could provide a basis for further research on 1-DNJ-producing bacteria.

Keywords

Bacillus velezensis
1-deoxynojirimycin
Genome
Isolation
α-Glucosidase inhibitor

Cited by (0)

1

Hyunjin Lee and Dong-Hyun Jung contributed equally to this work and should be considered co-first authors.

2

Won-Hyong Chung and Myung-Ji Seo contributed equally to this work and should be considered co-corresponding authors.