This study describes the first isolation and genetic characterization of the newly emerging porcine circovirus type 2e (PCV2e) from Korean pigs. The PCV2e isolate did not produce a cytopathic effect in PCV-free PK-15 cells; therefore, PCV2e infection was demonstrated by immunohistochemistry with polyclonal PCV2a antibodies and polymerase chain reaction with primers specific for PCV2e. As the infected PCV-free PK-15 cells were passaged, the amount of infectious virus correlated with an increase in the amount of viral DNA (i.e., a decrease in the cycle threshold values. A full genomic analysis of the PCV2e strain SNUVR199711 was performed and showed that the genome is 1,777 nucleotides in length.

这项研究描述了来自韩国猪的新出现的2e型猪圆环病毒（PCV2e）的首次分离和遗传特征。PCV2e分离株在不含PCV的PK-15细胞中未产生细胞病变作用；因此，通过多克隆PCV2a抗体的免疫组织化学和与PCV2e特异性引物的聚合酶链反应证明了PCV2e感染。由于被感染的自由PCV-PK-15细胞传代，感染性病毒的量的增加病毒DNA的量相关（我。ê中，在周期的阈值的降低。在PCV2e菌株的全基因组分析进行了SNUVR199711，结果表明基因组的长度为1,777个核苷酸。