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The first isolation of porcine circovirus type 2e from a Korean pig

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Abstract

This study describes the first isolation and genetic characterization of the newly emerging porcine circovirus type 2e (PCV2e) from Korean pigs. The PCV2e isolate did not produce a cytopathic effect in PCV-free PK-15 cells; therefore, PCV2e infection was demonstrated by immunohistochemistry with polyclonal PCV2a antibodies and polymerase chain reaction with primers specific for PCV2e. As the infected PCV-free PK-15 cells were passaged, the amount of infectious virus correlated with an increase in the amount of viral DNA (i.e., a decrease in the cycle threshold values. A full genomic analysis of the PCV2e strain SNUVR199711 was performed and showed that the genome is 1,777 nucleotides in length.

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Acknowledgements

The authors’ research was supported by contract research funds of the Research Institute for Veterinary Science (RIVS) from the College of Veterinary Medicine and by the BK 21 Plus Program (Grant no. 5260-20150100) for Creative Veterinary Science Research.

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Authors and Affiliations

  1. Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea

    Kee Hwan Park, Taehwan Oh, Hyejean Cho, Siyeon Yang & Chanhee Chae

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  1. Kee Hwan Park
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  2. Taehwan Oh
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Correspondence to Chanhee Chae.

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Park, K.H., Oh, T., Cho, H. et al. The first isolation of porcine circovirus type 2e from a Korean pig. Arch Virol 165, 2927–2930 (2020). https://doi.org/10.1007/s00705-020-04827-9

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  • DOI: https://doi.org/10.1007/s00705-020-04827-9

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