Peristenus spretus (Hymenoptera: Braconidae) is one of the most important endoparasitoids used for biological control of the green mirid bug, Apolygus lucorum Meyer-Dür (Heteroptera: Miridae). However, what we know about its reproductive genetics is very limited. Here, the composition of ovarian proteins in P. spretus was analyzed. Mass spectrum data searched against the non-redundant NCBI protein and UniProt protein database identified 1382 proteins and revealed an enrichment of the heat shock protein 83 (HSP83) in P. spretus ovary. The Pshsp83 complete cDNA is 2175 bp in length and encodes a protein of 724 amino acids with a calculated molecular mass of 83.4 kDa and a theoretical isoelectric point of 4.87. Transcription of Pshsp83 appeared from days 1 to 13 post-emergence and peaked at 13th day. Immuno-localization showed that the HSP83 protein was present in cytoplasm of germarium and in egg chambers of the whole ovariole. The transcript abundance of Pshsp83 fluctuated drastically after heat shocks at different temperatures and the maximum emerged at 35°C. The exposure to 35°C caused no dramatic effects on reproductive parameters of adult females such as pupation rate, cocoon weight, emergence rate, sex ratio and developmental duration, but did on longevity. These results suggested that the HSP83 protein is involved in life-span regulation in the P. spretus.

中文翻译：

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Peristenus spretus 卵巢的蛋白质组学研究和富含卵巢的热休克蛋白的表征

鲣鱼(膜翅目: Braconidae) 是最重要的内寄生蜂之一，用于生物防治绿蝽，芦荟Meyer-Dür（异翅目：Miridae）。然而，我们对其生殖遗传学的了解非常有限。在这里，卵巢蛋白质的组成P. spretus进行了分析。针对非冗余 NCBI 蛋白质和 UniProt 蛋白质数据库搜索的质谱数据鉴定了 1382 种蛋白质，并揭示了热激蛋白 83 (HSP83) 在P. spretus子房。这pshsp83完整的 cDNA 长度为 2175 bp，编码 724 个氨基酸的蛋白质，计算分子量为 83.4 kDa，理论等电点为 4.87。转录pshsp83出现在出苗后第 1 天至第 13 天，并在第 13 天达到峰值。免疫定位显示HSP83蛋白存在于germarium的细胞质和整个卵巢的卵室中。转录本丰度pshsp83不同温度的热冲击后波动剧烈，最大值出现在35°C。暴露于 35°C 对成年雌性的生殖参数（如化蛹率、茧重、出苗率、性别比和发育持续时间）没有显着影响，但对寿命有影响。这些结果表明，HSP83蛋白参与了生命周期的调控。P. spretus.