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Proteomic investigation of Peristenus spretus ovary and characterization of an ovary-enriched heat shock protein

Published online by Cambridge University Press:  30 September 2020

Miaomiao Tian Open the ORCID record for Miaomiao Tian [Opens in a new window] ,
Yayun Xia ,
Ducheng Cai ,
Jianjun Mao  and
Shuping Luo
Miaomiao Tian
Affiliation:
IPPCAAS – State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China College of Plant Protection, Hainan University, Hainan Haikou, China
Yayun Xia
Affiliation:
IPPCAAS – State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China College of Plant Protection, Hainan University, Hainan Haikou, China
Ducheng Cai
Affiliation:
College of Plant Protection, Hainan University, Hainan Haikou, China
Jianjun Mao*
Affiliation:
IPPCAAS – State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Shuping Luo*
Affiliation:
IPPCAAS – State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
*
Author for correspondence: Jianjun Mao, Email: maojianjun0615@126.com; Shuping Luo, Email: shp.l@126.com
Author for correspondence: Jianjun Mao, Email: maojianjun0615@126.com; Shuping Luo, Email: shp.l@126.com
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Abstract

Peristenus spretus (Hymenoptera: Braconidae) is one of the most important endoparasitoids used for biological control of the green mirid bug, Apolygus lucorum Meyer-Dür (Heteroptera: Miridae). However, what we know about its reproductive genetics is very limited. Here, the composition of ovarian proteins in P. spretus was analyzed. Mass spectrum data searched against the non-redundant NCBI protein and UniProt protein database identified 1382 proteins and revealed an enrichment of the heat shock protein 83 (HSP83) in P. spretus ovary. The Pshsp83 complete cDNA is 2175 bp in length and encodes a protein of 724 amino acids with a calculated molecular mass of 83.4 kDa and a theoretical isoelectric point of 4.87. Transcription of Pshsp83 appeared from days 1 to 13 post-emergence and peaked at 13th day. Immuno-localization showed that the HSP83 protein was present in cytoplasm of germarium and in egg chambers of the whole ovariole. The transcript abundance of Pshsp83 fluctuated drastically after heat shocks at different temperatures and the maximum emerged at 35°C. The exposure to 35°C caused no dramatic effects on reproductive parameters of adult females such as pupation rate, cocoon weight, emergence rate, sex ratio and developmental duration, but did on longevity. These results suggested that the HSP83 protein is involved in life-span regulation in the P. spretus.

Keywords

Heat shock proteinhsp83longevityOvary proteomePeristenus spretus
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 3 , June 2021 , pp. 270 - 281
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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