In this study, a micro-patterned hydroxyapatite/silk fibroin (HA-SF) coating was firstly fabricated on the surface of Mg-Zn-Y-Nd-Zr alloy by template-assisted electrospraying technique coupling with spin coating technique. Two types of micro-patterns were achieved with high contour accuracy, namely HA-SF(line-pattern) and HA-SF(dot-pattern). The microstructure, composition, surface wettability and corrosion behaviors of the coatings were investigated by SEM, EDS, FTIR, XRD, water contact angle and potentiodynamic polarization test. The results revealed the hydrophilic nature of coatings and two orders of magnitude reduction of corrosion density (i_corr) as compared with that of the substrate. All the micro-patterned surfaces promoted the attachment of MC3T3-E1 cells with visible filopodia after 1 d incubation. In addition, coatings with line pattern exhibited the superior guidance to cell migration as compared to dot pattern, and the preference of cell attachment in the convex zone was observed. In summary, the obtained micro-patterned HA-SF coatings possessed the remarkably improvement of anticorrosion ability and good efficacy in guidance of cell attachment and alignment, which can serve as a promising strategy for cellular response modulation at the interface of magnesium-based implants and bone.

在这项研究中，首先通过模板辅助电喷涂技术与旋涂技术相结合，在Mg-Zn-Y-Nd-Zr合金表面上制备了微图案化羟基磷灰石/丝素蛋白（HA-SF）涂层。两种具有高轮廓精度的微模式，即HA-SF（线型）和HA-SF（点型）。通过SEM，EDS，FTIR，XRD，水接触角和电位极化试验研究了涂层的微观结构，组成，表面润湿性和腐蚀行为。结果表明涂层具有亲水性，腐蚀密度降低了两个数量级（i _corr）与基材相比。孵育1 d后，所有具有微图案的表面均促进可见的丝状伪足促进MC3T3-E1细胞的附着。另外，与点状图案相比，具有线状图案的涂层对细胞迁移表现出更好的指导，并且观察到在凸区中细胞附着的偏好。综上所述，所获得的微细图案化的HA-SF涂层具有显着的抗腐蚀能力和良好的指导细胞附着和对准的功效，这可以作为在镁基植入物界面上调节细胞反应的有前途的策略。骨。