Abstract
In this study, a micro-patterned hydroxyapatite/silk fibroin (HA-SF) coating was firstly fabricated on the surface of Mg-Zn-Y-Nd-Zr alloy by template-assisted electrospraying technique coupling with spin coating technique. Two types of micro-patterns were achieved with high contour accuracy, namely HA-SF(line-pattern) and HA-SF(dot-pattern). The microstructure, composition, surface wettability and corrosion behaviors of the coatings were investigated by SEM, EDS, FTIR, XRD, water contact angle and potentiodynamic polarization test. The results revealed the hydrophilic nature of coatings and two orders of magnitude reduction of corrosion density (icorr) as compared with that of the substrate. All the micro-patterned surfaces promoted the attachment of MC3T3-E1 cells with visible filopodia after 1 d incubation. In addition, coatings with line pattern exhibited the superior guidance to cell migration as compared to dot pattern, and the preference of cell attachment in the convex zone was observed. In summary, the obtained micro-patterned HA-SF coatings possessed the remarkably improvement of anticorrosion ability and good efficacy in guidance of cell attachment and alignment, which can serve as a promising strategy for cellular response modulation at the interface of magnesium-based implants and bone.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (Grant Nos. 51601169, 51671175 and 51701184), the National Key Research and Development Program of China (2018YFC1106703) and the Key Projects of the Joint Fund of the National Natural Science Foundation of China (U1804251). Authors also would like to thank for the platformsupportfrom the Henan Key Laboratory of Advanced Magnesium Alloy and Key Laboratory of Materials Processing and Mold Technology (Ministry of Education).
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Chang, L., Li, X., Tang, X. et al. Micro-patterned hydroxyapatite/silk fibroin coatings on Mg-Zn-Y-Nd-Zr alloys for better corrosion resistance and cell behavior guidance. Front. Mater. Sci. 14, 413–425 (2020). https://doi.org/10.1007/s11706-020-0525-5
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DOI: https://doi.org/10.1007/s11706-020-0525-5