Key message

SlMYB75 increased the accumulation of JA and improved the scavenging of excess H ₂ O ₂ to resist B. cinerea. Overexpression of SlMYB75 greatly prolongs tomato fruit storage life.

Abstract

Botrytis cinerea (B. cinerea) is a major threat to the production and storage life of tomato (Solanum lycopersicum) fruit around the world. SlMYB75 is an R2R3MYB transcription factor associated with the biosynthesis of anthocyanidin, but little is known about its function in the resistance of tomato to B. cinerea. In this study, we found that the overexpression of SlMYB75 regulated the accumulation of jasmonic acid (JA) and promoted the JA-mediated signaling pathway to resist B. cinerea infection. Moreover, the activities of peroxidase and superoxide dismutase, which were activated to scavenge hydrogen peroxide produced as a result of the B. cinerea infection, were enhanced in the transgenic tomato plants. Scanning electron microscopy images showed that the wax on the fruit skin surface was significantly decreased in the transgenic tomatoes compared with the wild type. However, SlMYB75 prolonged fruit storage life by both enhancing resistance to B. cinerea and directly downregulating the fruit shelf life-related gene SlFSR. Collectively, this study provides a good candidate gene for breeding high-quality tomatoes with a long storage life and high disease resistance.

中文翻译：

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SlMYB75过表达增强番茄对灰霉病菌的抗性并延长果实贮藏期

关键信息

SlMYB75增加了JA的积累并提高了对过量H ₂ O ₂的清除以抵抗灰霉病菌。SlMYB75的过表达大大延长了番茄果实的贮藏期。

抽象的

Botrytis cinerea ( B. cinerea ) 是全球番茄 ( Solanum lycopersicum ) 果实生产和贮藏期的主要威胁。SlMYB75是一种与花青素生物合成相关的R2R3MYB转录因子，但对其在番茄对灰霉病菌抗性中的作用知之甚少。在本研究中，我们发现SlMYB75的过表达调节了茉莉酸 (JA) 的积累，并促进了 JA 介导的信号通路来抵抗B. cinerea感染。此外，在转基因番茄植株中，过氧化物酶和超氧化物歧化酶的活性被激活以清除由于B. cinerea感染而产生的过氧化氢。扫描电镜图像显示，与野生型相比，转基因番茄果皮表面的蜡质明显减少。然而，SlMYB75通过增强对B. cinerea的抗性和直接下调水果保质期相关基因SlFSR来延长水果贮藏期。综上所述，本研究为培育具有长贮藏期和高抗病性的优质番茄提供了良好的候选基因。